# US 12,000,005 B2 · Polynucleotide for identifying the sex of a Ginkgo biloba plant and use thereof

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- Number: US 12,000,005 B2
- Document ID: US12000005B2
- Status: Granted
- Kind: B2
- Application number: 17/619,970
- Filed: 2021-12-16
- Published: 2022-11-17
- Granted: 2024-06-04
- Earliest claimed: 2019-06-21
- Assignees: Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences; Agricultural Genomics Institute At Shenzhen, Chinese Academy of Agricultural Sciences
- Applicants: Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences
- Inventors: Jianbin Yan; Qinggang Liao; Junbo Gou; Ran Du; Sanwen Huang
- Web page: https://patentgrep.com/patent/US12000005B2

## CPC

- C12Q 1/6895: for plants, fungi or algae
- A01H 1/045: {using molecular markers}
- A01H 5/12: Leaves
- A01H 7/00: Gymnosperms, e.g. conifers
- C12Q 1/6844: Nucleic acid amplification reactions
- C12Q 1/686: Polymerase chain reaction [PCR]
- C12Q 2600/13: Plant traits
- C12Q 2600/158: Expression markers
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

Provided is primers, where the primers specifically bind to a polynucleotide and are used to amplify the polynucleotide; and the polynucleotide comprises or consists of the following sequence: 1) the nucleotide sequence represented by SEQ ID NO: 5; or 2) the complete complementary sequence of the sequence represented by SEQ ID NO: 5; or 3) a nucleotide sequence being identical to 300 bp or more contiguous nucleotides in the sequence of the above 1) or 2), where the nucleotide sequence being identical to 300 bp or more contiguous nucleotides is unique to a male Ginkgo biloba plant; and the sequences of the primers are respectively represented by SEQ ID NO: 1 and SEQ ID NO: 2.

## Claims

### Claim 1

Primers, wherein the primers specifically bind to a sequence of SEQ ID NO: 5 and produce a 1030 bp length PCR product; and the sequences of the primers are respectively represented by SEQ ID NO: 1 and SEQ ID NO: 2.