# US 11,999,990 B2 · Method for assaying the activity of spermidine/spermine N1-acetyltransferase

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- Number: US 11,999,990 B2
- Document ID: US11999990B2
- Status: Granted
- Kind: B2
- Application number: 17/895,690
- Filed: 2022-08-25
- Published: 2023-03-09
- Granted: 2024-06-04
- Earliest claimed: 2011-11-16
- Assignees: BioMark Technologies Inc.
- Applicants: BioMark Technologies Inc.
- Inventors: Brian Cheng; Rashid Bux; Daniel Sitar
- Web page: https://patentgrep.com/patent/US11999990B2

## CPC

- C12Q 1/48: involving transferase
- G01N 33/57557: {of other specific parts of the body, e.g. brain}
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A method for assaying activity of the enzyme spermidine/spermine N1-acetyltransferase (SSAT) uses SSAT substrates by detecting their acetylated forms. SSAT substrates may include rimantadine and tocainide wherein their metabolism occurs in part by the action of the inducible enzyme SSAT to produce the acetylated metabolites N-acetylrimantadine and N-acetyltocainide respectively. SSAT activity may be correlated to pathologic conditions.

## Claims

### Claim 1

A method for determining activity of spermine/spermidine N1-acetyltransferase (SSAT) in a mammal, the method comprising:
(a) providing a biological sample from the mammal obtained after an administration of a dose of rimantadine, wherein the biological sample comprises a detectable level of N-acetyl rimantadine; and
(b) quantifying the detectable level of N-acetyl rimantadine in the biological sample, thereby determining SSAT activity in the mammal.

### Claim 2 (depends on 1)

The method of claim 1, wherein the biological sample is blood.

### Claim 3 (depends on 1)

The method of claim 1, wherein the biological sample is urine.

### Claim 4 (depends on 1)

The method of claim 1, wherein the biological sample is saliva.

### Claim 5

A method for determining activity of spermine/spermidine N1-acetyltransferase (SSAT) in a mammal, the method comprising:
(a) administering a dose of rimantadine to the mammal;
(b) collecting a biological sample from the mammal following the administration of the dose of rimantadine, wherein the biological sample has a detectable level of N-acetyl rimantadine; and
(c) quantifying the detectable level of N-acetyl rimantadine in the biological sample, thereby determining SSAT activity in the mammal.

### Claim 6 (depends on 5)

The method of claim 5, wherein the biological sample is blood.

### Claim 7 (depends on 5)

The method of claim 5, wherein the biological sample is urine.

### Claim 8 (depends on 5)

The method of claim 5, wherein the biological sample is saliva.