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US 11,999,988 B2Granted
Method of increasing lipid productivity in nannochloropsis by introducing a gene encoding both a thioredoxin domain and a thioredoxin reductase domain
AssigneeKao Corporation
InventorTatsuro Ozaki
Life of this patent
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Abstract
A method of producing lipids, containing the steps of: culturing an alga in which expression of a gene encoding a protein containing a thioredoxin domain and a thioredoxin reductase domain is enhanced, and producing fatty acids or lipids containing the same as components.
Claims18
A method of producing lipids in a Nannochloropsis host, comprising the steps of:
- (a) introducing a gene encoding a protein containing a thioredoxin domain and a thioredoxin reductase domain into a Nannochloropsis host,
- (b) culturing the host in a suitable culture medium and at a suitable temperature and,
- (c) expressing the gene encoding the thioredoxin domain and the thioredoxin reductase domain in the host during the culturing, thereby producing fatty acids or lipids containing the same as components,
- wherein the thioredoxin domain is a domain consisting of the following amino acid sequence (A) or (B), or the following amino acid sequence (C) or (D), and the thioredoxin reductase domain is a domain consisting of the following amino acid sequence (E) or (F), or the following amino acid sequence (G) or (H):
- (A) the amino acid sequence at positions 529 to 629 of the amino acid sequence set forth in SEQ ID NO:
- 1;
- (B) an amino acid sequence having 90% or more identity with the amino acid sequence (A), and constituting the thioredoxin domain having thioredoxin activity;
- (C) the amino acid sequence at positions 525 to 625 of the amino acid sequence set forth in SEQ ID NO:
- 3;
- (D) an amino acid sequence having 90% or more identity with the amino acid sequence (C), and constituting the thioredoxin domain having thioredoxin activity;
- (E) the amino acid sequence at positions 137 to 448 of the amino acid sequence set forth in SEQ ID NO:
- 1;
- (F) an amino acid sequence having 90% or more identity with the amino acid sequence (E), and constituting the thioredoxin reductase domain having thioredoxin reductase activity;
- (G) the amino acid sequence at positions 134 to 445 of the amino acid sequence set forth in SEQ ID NO:
- 3;
- and
- (H) an amino acid sequence having 90% or more identity with the amino acid sequence (G), and constituting the thioredoxin reductase domain having thioredoxin reductase activity.
14 more elements
A method of improving lipid productivity in a Nannochloropsis host, comprising
- (a) introducing a gene encoding a protein containing a thioredoxin domain and a thioredoxin reductase domain into a Nannochloropsis host,
- (b) culturing the Nannochloropsis host in a suitable culture medium and at a suitable temperature and,
- (c) expressing the gene encoding the thioredoxin domain and the thioredoxin reductase domain in the Nannochloropsis host during the culturing, thereby improving the lipid productivity of the Nannochloropsis host into which the gene has been introduced as compared to that of the Nannochloropsis host into which the gene has not been introduced,
- wherein the thioredoxin domain is a domain consisting of the following amino acid sequence (A) or (B), or the following amino acid sequence (C) or (D), and the thioredoxin reductase domain is a domain consisting of the following amino acid sequence (E) or (F), or the following amino acid sequence (G) or (H):
- (A) the amino acid sequence at positions 529 to 629 of the amino acid sequence set forth in SEQ ID NO:
- 1;
- (B) an amino acid sequence having 90% or more identity with the amino acid sequence (A), and constituting the thioredoxin domain having thioredoxin activity;
- (C) the amino acid sequence at positions 525 to 625 of the amino acid sequence set forth in SEQ ID NO:
- 3;
- (D) an amino acid sequence having 90% or more identity with the amino acid sequence (C), and constituting the thioredoxin domain having thioredoxin activity;
- (E) the amino acid sequence at positions 137 to 448 of the amino acid sequence set forth in SEQ ID NO:
- 1;
- (F) an amino acid sequence having 90% or more identity with the amino acid sequence (E), and constituting the thioredoxin reductase domain having thioredoxin reductase activity;
- (G) the amino acid sequence at positions 134 to 445 of the amino acid sequence set forth in SEQ ID NO:
- 3;
- and
- (H) an amino acid sequence having 90% or more identity with the amino acid sequence (G), and constituting the thioredoxin reductase domain having thioredoxin reductase activity.
14 more elements
A transformant of an alga, wherein the transformant comprises a gene encoding a protein containing a thioredoxin domain and a thioredoxin reductase domain that has been introduced into the transformant, thereby enhancing expression of the gene in the transformant.
Dependencies are inferred from the claim text.