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US 11,999,983 B2Granted
Method for producing basic L-amino acids or salts thereof by fermentation of an Enterobacteriaceae bacterium
AssigneeAjinomoto Co., Inc.
InventorsMikhail Kharisovich Ziyatdinov, Yulia Georgievna Rostova, Olga Nikolaevna Igonina, Andrei Viacheslavovich Kniazev
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Abstract
The present invention provides a method for producing a basic L-amino acid, for example, L-ornithine L-citrulline, L-arginine, L-histidine, and L-lysine by fermentation using a bacterium belonging to the family Enterobacteriaceae which has been modified to overexpress a gene encoding a protein having L-methionine/branched chain amino acid exporter activity.
Claims5
A method for producing a basic L-amino acid comprising:
- (i) cultivating a basic L-amino acid producing bacterium belonging to the family Enterobacteriaceae in a culture medium to produce and accumulate the basic L-amino acid in the culture medium or cells of the bacterium, or both, and
- (ii) collecting the basic L-amino acid from the culture medium or the cells, or both,
- wherein said bacterium has been modified to overexpress a DNA encoding a protein having L-methionine/branched chain amino acid exporter activity, and
- wherein said protein having L-methionine/branched chain amino acid exporter activity is selected from the group consisting of:
- (A) a YjeH protein,
- (B) a protein comprising the amino acid sequence set forth in SEQ ID NO:
- 2,
- (C) a protein comprising the amino acid sequence set forth in SEQ ID NO:
- 2, but which consists of substitution, deletion, insertion, and/or addition of 1 to 40 amino acid residues, and wherein said protein has L-methionine/branched chain amino acid exporter activity, and
- (D) a protein comprising an amino acid sequence that is at least 90% identical to the amino acid sequence set forth in SEQ ID NO:
- 2, and wherein said protein has L-methionine/branched chain amino acid exporter activity;
- or
- wherein said protein having L-methionine/branched chain amino acid exporter activity is encoded by a DNA selected from the group consisting of:
- (a) a yjeH gene,
- (b) a DNA comprising the nucleotide sequence set forth in SEQ ID NO:
- 1,
- (c) a DNA encoding a protein comprising the amino acid sequence set forth in SEQ ID NO:
- 2, but which consists of substitution, deletion, insertion and/or addition of 1 to 40 amino acid residues, and wherein said protein has L-methionine/branched chain amino acid exporter activity,
- (d) a DNA encoding a protein comprising an amino acid sequence that is at least 90% identical to the amino acid sequence set forth in SEQ ID NO:
- 2, and wherein said protein has L-methionine/branched chain amino acid exporter activity, and
- (e) a DNA which is a variant nucleotide sequence of SEQ ID NO:
- 1 due to the degeneracy of the genetic code.
19 more elements
Dependencies are inferred from the claim text.