# US 11,999,979 B2 · Lachnospiraceae sp. CAS12A mutants with enhanced cleavage activity at non-canonical TTTT protospacer adjacent motifs

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 11,999,979 B2
- Document ID: US11999979B2
- Status: Granted
- Kind: B2
- Application number: 17/245,401
- Filed: 2021-04-30
- Published: 2021-11-11
- Granted: 2024-06-04
- Earliest claimed: 2020-05-01
- Assignees: Integrated Dna Technologies, Inc.
- Applicants: Integrated Dna Technologies, Inc.
- Inventors: Liyang Zhang; Christopher Anthony Vakulskas
- Web page: https://patentgrep.com/patent/US11999979B2

## CPC

- C12N 9/22: Ribonucleases {[RNase]; Deoxyribonucleases [DNase]}
- C12N 9/226: {Class 2 CAS enzyme complex, e.g. single CAS protein}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Described herein are CAS12A mutants from Lachnospiraceae bacterium and methods for use thereof. These mutants have enhanced DNA cleavage activities at non-canonical TTTT protospacer adjacent motifs (PAM) compared to the wild-type enzyme.

## Claims

### Claim 1

An isolated mutant Lachnospiraceae bacterium ND2006 Cas12a (“LbCas12a”) polypeptide comprising the G146R/R182V/E795Q polypeptide sequence of SEQ ID NO: 39844.

### Claim 2 (depends on 1)

An isolated polynucleotide sequence encoding the mutant LbCas12a polypeptide of claim 1, wherein the polynucleotide sequence comprises the sequence of SEQ ID NO: 3983.