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US 11,999,968 B2Granted
Modified T cells, pharmaceutical composition, manufacturing method thereof, and method of using the same
AssigneeFullhope Biomedical Co., Ltd.
InventorsJan-Mou Lee, Chun-Wei Yu, Chih-Hao Fang, Ya-Fang Cheng, Li-Tzong Chen
Life of this patent
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Abstract
This disclosure provides modified T cells possessing both cell killing function and antigen presenting cell function and method of culturing the same. By administration of the modified T cell, cancer cells in a subject may be effectively inhibited via cell-mediated immunity.
Claims9
A method of culturing a modified T cell, comprising obtaining a body fluid comprising mononuclear cells, wherein the mononuclear cells comprise T cells;
- contacting the mononuclear cells with an inducing culturing medium comprising IL-2 and zoledronic acid to obtain a first cultured cell population;
- contacting the first cultured cell population with a first culturing medium comprising IL-2 to obtain a second cultured cell population;
- contacting the second cultured cell population with a second culturing medium comprising IL-2 and IL-12 to obtain a third cultured cell population;
- and
- contacting the third cultured cell population with a third culturing medium comprising IL-2, IL-12 and IL-18 after contacting with the second culturing medium to obtain a fourth cultured cell population that comprises the modified T cells;
- wherein each of the inducing culturing medium, the first culturing medium, the second culturing medium, and the third culturing medium further comprises L-glutamine, streptomycin sulfate, gentamicin sulfate and human platelet lysate.
3 more elements
Dependencies are inferred from the claim text.