Bocaparvovirus small noncoding RNA and uses thereof
AssigneesUniversity of Iowa Research Foundation, University of Kansas
InventorsZiying Yan, John F. Engelhardt, Jianming Qiu, Zekun Wang
Life of this patent
Estimating the term…
Abstract
Vectors having a nucleotide sequence having SEQ ID NO:1 or a nucleotide sequence having at least 85% identity to SEQ ID NO:1, or a portion thereof, that is capable of regulating bocaparvovirus replication, or vectors having the complement of the nucleotide sequence, and methods of using the vectors, are provided.
Claims10
A helper virus-free vector system to produce recombinant adeno-associated virus (rAAV) or to prepare helper virus-free chimeric rAAV/BoV, comprising:
- i) one or more rAAV vectors, and one or more BoV vectors that express one or more of BoV NP1, BoV NS4 or NS2 and a nucleotide sequence having SEQ ID NO:1 or a nucleotide sequence having at least 85% identity to SEQ ID NO:1, that is capable of regulating bocavirus replication, wherein the rAAV vector or the BoV vector or another vector in mammalian cells express AAV Rep and/or AAV Cap, wherein the cells and/or the vectors do not express BoV VP1-3,
- ii) one or more rAAY vectors, one or more BoV vectors that express one or more of BoV NP1, and BoV NS4 or NS2, and a vector that expresses at least adenovirus E2A, wherein the rAAV vector or the BoV vector or other vector in mammalian cells express AAV Rep and/or AAV Cap, wherein the cells and vectors do not express BoV VP1-3 or another viral capsid protein;
- or
- iii) one or more rAAV vectors, and one or more BoV vectors that express one or more of BoV NP1, BoV NS4 or NS2, and a nucleotide sequence having SEQ ID -NO:
- 1 or a nucleotide sequence having at least 85% identity to SEQ ID NO:1, that is capable of regulating bocavirus replication, wherein the vectors or cells do not express BoV NS1 or AAV Cap and wherein at least one of the rAAV vectors, the BoV vectors or cells expresses AAV Rep and/or Bov VP1-3.
An isolated mammalian cell for helper virus-free production of recombinant adeno-associated virus (rAAV), wherein the mammalian cell
- i) expresses a rAAV genome comprising a transgene, BoV NP1, BoV NS4 or NS2, a nucleotide sequence having SEQ ID NO:
- 1 or a nucleotide sequence having at least 85% identity to SEQ ID NO:1, that is capable of regulating bocavirus replication, and expresses AAV Rep and/or AAV Cap, or
- ii) expresses a rAAV genome comprising a transgene, one or more of BoV NP1, and BoV NS4 or NS2, adenovirus E2A, and AAV Rep and/or AAV Cap, in an amount effective to yield helper-free rAAV, wherein the mammalian cell does not express viral capsid protein other than AAV Cap.
Dependencies are inferred from the claim text.