# US 11,999,963 B2 · Pseudomonas protegens and products thereof to control bacterial panicle blight of rice

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- Number: US 11,999,963 B2
- Document ID: US11999963B2
- Status: Granted
- Kind: B2
- Application number: 17/341,498
- Filed: 2021-06-08
- Published: 2021-12-16
- Granted: 2024-06-04
- Earliest claimed: 2020-06-08
- Assignees: Board of Trustees of the University of Arkansas
- Applicants: The Board of Trustees of the University of Arkansas
- Inventors: Clemencia M. Rojas; J. Alejandro Rojas; Jackson O. Lay, Jr.
- Web page: https://patentgrep.com/patent/US11999963B2

## CPC

- A01N 63/27: Pseudomonas
- C12N 15/8281: {for bacterial resistance}
- A01P 1/00: Disinfectants; Antimicrobial compounds or mixtures thereof
- C07K 14/21: from Pseudomonadaceae (F)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01P: BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The present invention provides biosynthetic products that may be useful for controlling bacterial panicle blight of rice. These products are encoded by four biosynthetic operons found within the genome of a Pseudomonas protegens strain designated as PBL3 and deposited as ATCC #, which has been shown to inhibit the growth of Burkholderia glumae on rice plants. Also provided are vectors encoding these biosynthetic products, and methods of using the biosynthetic products to inhibit the growth of microorganisms.

## Claims

### Claim 1

A method of inhibiting the growth of Burkholderia glumae, the method comprising: contacting the Burkholderia glumae with an effective amount of a biosynthetic product, thereby inhibiting its growth, wherein the biosynthetic product is produced by a Pseudomonas protegens strain designated as PBL3 and deposited as NRRL Accession No. B-68083, and wherein the biosynthetic product is selected from orfamide A/C, pyoluteorin, and pyrrolnitrin.

### Claim 2 (depends on 1)

The method of claim 1, wherein the growth of the Burkholderia glumae is inhibited on a plant.

### Claim 3 (depends on 2)

The method of claim 2, wherein the plant is a rice plant.

### Claim 4 (depends on 2)

The method of claim 2, wherein the contacting is carried out by spraying or dusting the plant or a portion of the plant with a composition comprising the biosynthetic product.

### Claim 5 (depends on 2)

The method of claim 2, wherein the contacting is carried out before flowering or during panicle formation.