# US 11,999,957 B2 · Method for enhancing vanillin resistance of Saccharomyces cerevisiae by knocking out SNG1 gene

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- Number: US 11,999,957 B2
- Document ID: US11999957B2
- Status: Granted
- Kind: B2
- Application number: 17/118,179
- Filed: 2020-12-10
- Published: 2021-12-16
- Granted: 2024-06-04
- Earliest claimed: 2020-06-10
- Assignees: Qilu University of Technology
- Applicants: Qilu University of Technology
- Inventors: Xiaoming Bao; Liyun Song; Xinning Wang; Wenyan Cao; Weiquan Zhao; Zailu Li
- Web page: https://patentgrep.com/patent/US11999957B2

## CPC

- C12N 15/81: for yeasts
- C07K 14/395: from Saccharomyces
- C12N 1/36: Adaptation or attenuation of cells
- C12N 15/905: {in yeast}
- C12R 2001/865: Saccharomyces cerevisiae
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

A method of enhancing vanillin resistance of Saccharomyces cerevisiae, including: knocking out SNG1 gene from a genome of Saccharomyces cerevisiae. This application further provides a mutant of SNG1 gene of Saccharomyces cerevisiae including the nucleotide sequence shown in SEQ ID NO: 1, where the sequence shown in SEQ ID NO: 1, from left to right, consists of a −18˜+203 bp fragment of SNG1 gene of Saccharomyces cerevisiae, a nucleotide fragment of loxp-KanMX4-loxp and a +1446˜+1644 bp fragment of the SNG1 gene of Saccharomyces cerevisiae.

## Claims

### Claim 1

A mutant SNG1 gene, comprising the nucleotide sequence of SEQ ID NO: 1.

### Claim 2

A Saccharomyces cerevisiae cell with vanillin resistance, the Saccharomyces cerevisiae cell comprising a mutant SNG1 gene comprising the nucleotide sequence of SEQ ID NO: 1.