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US 11,999,945 B2Granted
Modulating polymer beads for DNA processing
AssigneeIllumina, Inc.
InventorsSteven Norberg, Dmitry K. Pokholok, Ramesh Ramji, Frank J. Steemers, Fan Zhang
Life of this patent
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Abstract
Systems, methods, and compositions provided herein relate to preparation of beads encapsulating biomolecules for performing sequential reactions on the biomolecules. Some embodiments include preparation of nucleic acid reactions within the bead, wherein the bead includes pores that allow diffusion of molecules into or out of the beads while retaining other molecules of interest.
Claims24
A solid support comprising a plurality of beads, wherein each bead comprises:
- a hydrogel polymer precursor;
- a crosslinker;
- and
- a biomolecule disposed within each bead, wherein each bead is a porous hollow bead that comprises multiple polymer layers, wherein each polymer layer comprises at least one of a distinct pore size and a distinct pore density that allows diffusion of a reagent through the bead while retaining the biomolecule.
1 more element
A method of preparing a nucleic acid library, comprising:
- introducing a plurality of beads to a flow cell device, wherein each bead comprises:
- a hydrogel polymer precursor;
- a crosslinker;
- and
- a biomolecule disposed within each bead, wherein the bead is a porous hollow bead that comprises multiple polymer layers, wherein each polymer layer comprises at least one of a distinct pore size and a distinct pore density that allows diffusion of a reagent through the bead while retaining the biomolecule,
- introducing nucleic acid library preparation enzymes to the flow cell device;
- forming a DNA library from the biomolecule within each bead;
- and
- releasing the DNA library onto the flow cell device.
6 more elements
Dependencies are inferred from the claim text.