Materials and methods for maximizing biosynthesis through alteration of pyruvate-acetyl-CoA-TCA balance in species of the genera ralstonia and cupriavidus and organisms related thereto

A method of redirecting carbon flux in an organism toward carbon-based chemical products having a C4/C5/C6 chain length, said method comprising overexpressing one or more pck gene encoding a phosphoenolpyruvate carboxykinase and/or pyc gene encoding a phosphoenolpyruvate carboxylase and/or ppc gene encoding a pyruvate carboxylase and one or more CitE genes and a CitF gene encoding a citryl-CoA lyase to increase an activity of one or more phosphoenolpyruvate carboxykinase having phosphoenolpyruvate carboxykinase activity and/or a phosphoenolpyruvate carboxylase having phosphoenolpyruvate carboxylase activity and/or a pyruvate carboxylase having pyruvate carboxylase activity and a citrate lyase or citrate lyase subunit as compared to an organism which does not overexpress one or more pck gene and/or pyc gene and/or ppc gene and one or more CitE genes and a CitF gene and fermenting the organism under glycolysis or mixotrophic conditions to redirect carbon flux in the organism toward products having a C4/C5/C6 chain length,

• wherein the phosphoenolpyruvate carboxykinase comprises at least 90% amino acid sequence identity the to the amino acid sequence of SEQ ID NO:
• 8, 12, 14, 16, or 18;
• the phosphoenolpyruvate carboxylase comprises at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO:
• 32, 34, 36, 38, or 40;
• the pyruvate carboxylase comprises at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO:
• 26 or 28;
• the citrate lyase or citrate lyase subunit comprises at least 90% amino acid sequence identity the amino acid sequence of SEQ ID NO:
• 44, 46, 48, 50, and/or 52;
• and
• wherein the organism is Cupriavidus necator and wherein said Cupriavidus necator comprises diminished polyhydroxybutyrate synthesis as a result of said overexpression as compared to an unperturbed wild-type Cupravidus necator organism.