# US 11,999,941 B2 · Composition suitable for protecting microorganisms

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 11,999,941 B2
- Document ID: US11999941B2
- Status: Granted
- Kind: B2
- Application number: 17/957,224
- Filed: 2022-09-30
- Published: 2023-02-02
- Granted: 2024-06-04
- Earliest claimed: 2015-06-30
- Assignees: Societe des Produits Nestle S.A.
- Applicants: SociéTé Des Produits Nestlé S.A.
- Inventors: Edwin Ananta; Biljana Bogicevic; Carsten Cramer; Alexandra Dubois; Judith Gaulocher; Katja Johnson; Jeroen Andre Muller; Matthias Perren; Guenolee Eliane Marie Prioult; Sabine Sres; Wilbert Sybesma
- Web page: https://patentgrep.com/patent/US11999941B2

## CPC

- C12N 1/04: Preserving or maintaining viable microorganisms (immobilised microorganisms C12N11/00)
- C12N 1/20: Bacteria; Culture media therefor
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to a composition comprising at least one carrier comprising a polysaccharide, at least one antioxidant and at least one amino acid selected from cysteine, lysine, alanine and arginine. It also relates to the use of such a composition for the protection of microorganisms during drying, storage and/or reconstitution, to a culture powder, to a process of making the culture powder and to products comprising the culture powder.

## Claims

### Claim 1

A culture powder composition comprising live microorganisms and a matrix comprising a polysaccharide, at least one antioxidant and at least one amino acid combination selected from the group consisting of;
(i) cysteine that is 2 to 10 wt % of total dry weight of the composition, and alanine that is 8 to 20 wt % of total dry weight of the composition;
(ii) cysteine that is 2 to 10 wt % of total dry weight of the composition, and lysine and alanine that are each 8 to 20 wt % of total dry weight of the composition;
(iii) lysine and arginine that are each 8 to 20 wt % of total dry weight of the composition;
(iv) cysteine that is 2 to 10 wt % of total dry weight of the composition, and arginine that is 8 to 20 wt % of total dry weight of the composition;
(v) cysteine that is 2 to 10 wt % of total dry weight of the composition, and lysine and arginine that are each 8 to 20 wt % of total dry weight of the composition;
(vi) lysine, alanine, and arginine that are each 8 to 20 wt % of total dry weight of the composition; and
(vii) cysteine that is 2 to 10 wt % of total dry weight of the composition, and arginine and alanine that are each 8 to 20 wt % of total dry weight of the composition,
(viii) wherein a dry weight ratio of the matrix:the live microorganisms is at least 1.

### Claim 2 (depends on 1)

The culture powder according to claim 1, wherein the live microorganisms are bacteria.

### Claim 3 (depends on 2)

The culture powder according to claim 2, wherein the bacteria is selected from the group consisting of bifidobacteria, lactobacilli, lactococci, enterococci, streptococci, Leuconostoc, Escherichia, propionibacteria, and combinations thereof.