# US 11,999,935 B2 · Bioreactor for use on a moving platform, bioreactor motion system, and method of performing a bioprocess using a bioreactor motion system > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,999,935 B2 - Document ID: US11999935B2 - Status: Granted - Kind: B2 - Application number: 16/857,296 - Filed: 2020-04-24 - Published: 2020-10-29 - Granted: 2024-06-04 - Earliest claimed: 2019-04-24 - Assignees: Sartorius Stedim Biotech GmbH - Applicants: Sartorius Stedim Biotech GmbH - Inventors: Gregory Bremer; Clint Kukla; Thorsten Adams; Fabian Tunzini; Rachel Delessert; Michael Bates - Web page: https://patentgrep.com/patent/US11999935B2 ## CPC - C12M 27/16: {Vibrating; Shaking; Tilting} - C12M 23/14: {Bags} - C12M 23/26: {flexible (flexible containers for laboratory use B01L3/505)} - C12M 29/10: {Perfusion} - C12M 33/22: {Settling tanks; Sedimentation by gravity (settling tanks per se B01D21/02)} - C12M 41/00: {Means for regulation, monitoring, measurement or control, e.g. flow regulation (controlling or regulating chemical, physical or physicochemical processes B01J19/0006; heating or cooling apparatus for laboratory use B01L7/00; electro optical investigation of individual particles, flow cytometers G01N15/14; automatic analysis G01N35/00; controlling or regulating in general G06N)} - C12M 41/48: {Automatic or computerized control (automatic analysis G01N35/00)} - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract A bioreactor for use on a moving platform including a bag. According to a first aspect, the bag defines a three-dimensional geometrical shape including at least one lower collecting vertex in which at least two edges of the geometrical shape meet at an angle not greater than 90°. The bioreactor further includes at least one port or sensor arranged in the vicinity of the collecting vertex. According to a second aspect, the bag defines a two-dimensional geometrical shape or a three-dimensional geometrical shape. The geometrical shape includes at least one edge, in which at least two faces of the geometrical shape meet at an angle not greater than 90°. A longitudinal port/sensor element is arranged along the edge and includes several ports and/or sensors. A bioreactor motion system including a moving platform and a bioreactor according to the first or second aspect. A method of performing a bioprocess. ## Claims ### Claim 1 A bioreactor motion system, comprising: a moving platform; a bioreactor comprising a bag which defines a three-dimensional geometrical shape including a bottom with respect to a use position on the moving platform and a lower collecting vertex located at the bottom where at least two edges of the three-dimensional geometrical shape meet at an angle, wherein a working volume of the bag is in a range of 50 to 1000 mL; and a longitudinal port/sensor element arranged along a first one of the at least two edges, the longitudinal port/sensor element including several ports and/or sensors arranged as a single unit, wherein the longitudinal port/sensor element extends, with respect to its longitudinal direction, along the first one of the at least two edges of the three-dimensional geometrical shape of the bag, the first one of the at least two edges extending from the lower collecting vertex in a direction away from the bottom, such that, when the bioreactor is in the use position on the moving platform, the longitudinal port/sensor element extends away from the moving platform. ### Claim 2 (depends on 1) The bioreactor motion system according to claim 1, characterized in that the angle where the at least two edges of the three-dimensional geometrical shape meet is not greater than 90°. ### Claim 3 (depends on 1) The bioreactor motion system according to claim 1, characterized in that the bioreactor is a single-use bioreactor with the bag being made from a flexible film material. ### Claim 4 (depends on 3) The bioreactor motion system according to claim 3, characterized in that the longitudinal port/sensor element is welded between opposite edges of the flexible film material from which the bag is made. ### Claim 5 (depends on 1) The bioreactor motion system according to claim 1, characterized in that the longitudinal port/sensor element is oriented vertically in the use position of the bioreactor. ### Claim 6 (depends on 1) The bioreactor motion system according to claim 1, characterized in that one or more ports of the longitudinal port/sensor element are used to connect at least one of: a sensor; an input line; an output line; an inlet filter; an outlet filter. ### Claim 7 (depends on 1) The bioreactor motion system according to claim 1, characterized in that at least one other port is provided in an upper face of the bag. ### Claim 8 (depends on 1) The bioreactor motion system according to claim 1, characterized in that a perfusion membrane is arranged at the bottom of the bag, remote from the longitudinal port/sensor element, and forms a pocket inside the bag, the bioreactor further comprising a perfusion harvest line or a perfusion harvest port opening through a bottom wall of the bottom of the bag into the pocket. ### Claim 9 (depends on 8) The bioreactor motion system according to claim 8, characterized in that the perfusion membrane is located in a vicinity of a lower vertex or an edge of the bag remote from the lower collecting vertex or the longitudinal port/sensor element. ### Claim 10 (depends on 1) The bioreactor motion system according to claim 1, characterized in that the bag defines the three-dimensional geometrical shape of a tetrahedron. ### Claim 11 (depends on 1) The bioreactor motion system according to claim 1, wherein the moving platform is a rocking motion platform on which the bottom of the bioreactor is placed adjacent to the moving platform. ### Claim 12 (depends on 11) The bioreactor motion system according to claim 11, characterized in that the moving platform is equipped with an auxiliary construction including means for altering an effective tilting angle range of a surface on which the bioreactor is to be placed. ### Claim 13 (depends on 11) The bioreactor motion system according to claim 11, characterized in that the moving platform is equipped with an auxiliary construction providing a passage to allow a perfusion harvest line to be connected from underneath the bag. ### Claim 14 (depends on 11) The bioreactor motion system according to claim 11, further comprising at least one support to prevent the bioreactor from shifting or tipping over on the moving platform, the at least one support including a rail or a recess. ### Claim 15 (depends on 11) The bioreactor motion system according to claim 11, characterized in that the moving platform is equipped with a load cell. ### Claim 16 (depends on 11) A method of performing a bioprocess, comprising steps of: providing the bioreactor motion system according to claim 11; filling the bioreactor with cell cultivation liquid; placing the bioreactor on the moving platform with the bottom of the bioreactor adjacent to the moving platform; and moving the moving platform repeatedly with a maximum tilting angle which is controlled by a control unit. ### Claim 17 (depends on 16) The method according to claim 16, characterized in that the control unit adjusts the maximum tilting angle depending on a current volume of the cell cultivation liquid contained in the bioreactor. ### Claim 18 (depends on 17) The method according to claim 17, characterized in that a weight of the cell cultivation liquid contained in the bioreactor is automatically measured, and the current volume of the cell cultivation liquid contained in the bioreactor is calculated from the weight of the cell cultivation liquid. ### Claim 19 (depends on 1) The bioreactor motion system according to claim 1, wherein the longitudinal port/sensor element extends obliquely upwards from the at least one lower collecting vertex but does not reach an opposite vertex at an opposite end of the first one of the at least two edges.