# US 11,999,933 B2 · Biological sheet culture device and biological sheet culture facility > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,999,933 B2 - Document ID: US11999933B2 - Status: Granted - Kind: B2 - Application number: 16/757,403 - Filed: 2018-10-08 - Published: 2021-06-24 - Granted: 2024-06-04 - Earliest claimed: 2018-02-13 - Assignees: Boe Technology Group Co., Ltd. - Applicants: Boe Technology Group Co., Ltd. - Inventors: Shi Zhou - Web page: https://patentgrep.com/patent/US11999933B2 ## CPC - C12M 23/10: {Petri dish (crystallising dishes B01L3/06)} - C12M 25/14: {Scaffolds; Matrices (in general C12N5/0068)} - C12M 23/22: {Transparent or translucent parts (glassware for laboratory use B01L3/00)} - C12M 23/24: {Gas permeable parts} - C12M 23/48: {Holding appliances; Racks; Supports (holding devices for laboratory apparatus B01L9/00)} - C12M 23/58: {Reaction vessels connected in series or in parallel (combinations of bioreactors with other apparatus, C12M43/00)} - C12M 27/18: {Flow directing inserts} - C12M 29/04: {Filters; Permeable or porous membranes or plates, e.g. dialysis} - C12M 29/10: {Perfusion} - C12M 29/18: {External loop; Means for reintroduction of fermented biomass or liquid percolate (loop type reactors for chemical or physical processes B01J19/2435)} - C12M 29/26: {Conditioning fluids entering or exiting the reaction vessel} - C12M 41/14: {Incubators; Climatic chambers (per se B01L1/00)} - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract A biological sheet culture device includes a scaffold, a number of culture dishes for culturing biological sheets positioned on the scaffold and at least one fluid channel configured to traverse each culture dish. ## Claims ### Claim 1 A biological sheet culture device comprising: a scaffold; a plurality of culture dishes for culturing biological sheets on the scaffold; and a plurality of communication pipes, each of which is configured to fluidly communicate with two adjacent culture dishes; wherein the plurality of culture dishes and the plurality of communication pipes are configured to form a plurality of fluid channels that are continuous, each fluid channel is configured to traverse every culture dish of the plurality of culture dishes, a sequence of one fluid channel of the plurality of fluid channels flowing through the plurality of culture dishes is different from that of at least one other fluid channel of the plurality of fluid channels flowing through the plurality of culture dishes wherein the plurality of fluid channels intersect each other through part of the plurality of culture dishes, wherein the plurality of fluid channels are configured such that adding culture medium to any single culture dish of the plurality of fluid channels can cause the culture medium to flow into all other culture dishes of the plurality of fluid channels, wherein an air permeable pipe is provided in a culture dish of the plurality of culture dishes, the air permeable pipe is configured to communicate from inside of the culture dish to outside of the culture dish, a permeable film is provided in the permeable pipe and configured to prevent dust and impurities from entering the culture dish through the air permeable pipe and allow air to enter the culture dish, and wherein the biological sheet culture device further comprises a water permeable pipe that extends into a culture dish of the plurality of culture dishes from a top of the culture dish of the plurality of culture dishes, and a fiber film is provided in the water permeable pipe and configured to prevent substances other than the culture medium in the culture dish from being discharged out of the culture dish through the water permeable pipe. ### Claim 2 (depends on 1) The biological sheet culture device according to claim 1, wherein at least two of the culture dishes are configured to be on the scaffold at different heights. ### Claim 3 (depends on 2) The biological sheet culture device according to claim 2, wherein two adjacent culture dishes with different heights are configured to be communicated through a communication pipe, and wherein a portion of a culture medium in a culture dish at a higher position of the two adjacent culture dishes is configured to flow into a culture dish at a lower position of the two adjacent culture dishes through the communication pipe under a state that the culture dish at the higher position includes the culture medium. ### Claim 4 (depends on 3) The biological sheet culture device according to claim 3, wherein at least portions of two culture dishes communicating with at least one culture dish are at a same side of a longitudinal direction of the at least one culture dish. ### Claim 5 (depends on 3) The biological sheet culture device according to claim 3, wherein at least portions of two culture dishes communicating with at least one culture dish are at both sides of a longitudinal direction of the at least one culture dish. ### Claim 6 (depends on 3) The biological sheet culture device according to claim 3, further comprising: a circulation pipe connected to culture dishes of different heights and configured to return at least part of a culture medium in a culture dish at a lower position into a culture dish at a higher position. ### Claim 7 (depends on 2) The biological sheet culture device according to claim 2, wherein a guide plate is between two adjacent culture dishes with different heights, and wherein a portion of a culture medium in a culture dish at a higher position of the two adjacent culture dishes is configured to flow into a culture dish at a lower position of the two adjacent culture dishes through the guide plate under a state that the culture dish at the higher position includes the culture medium. ### Claim 8 (depends on 7) The biological sheet culture device according to claim 7, wherein at least portions of two culture dishes communicating with at least one culture dish are at a same side of a longitudinal direction of the at least one culture dish. ### Claim 9 (depends on 7) The biological sheet culture device according to claim 7, wherein at least portions of two culture dishes communicating with at least one culture dish are at both sides of a longitudinal direction of the at least one culture dish. ### Claim 10 (depends on 2) The biological sheet culture device according to claim 2, further comprising: a circulation pipe connected to culture dishes of different heights and configured to return at least part of a culture medium in a culture dish at a lower position into a culture dishes at a higher position. ### Claim 11 (depends on 10) The biological sheet culture device according to claim 10, further comprising: a liquid supply assembly, wherein the liquid supply assembly comprises a first pump and a liquid supply pipe connected with the first pump, and wherein the first pump supplements the culture medium into the circulation pipe through the liquid supply pipe. ### Claim 12 (depends on 10) The biological sheet culture device according to claim 10, wherein the circulation pipe is associated with a switching valve. ### Claim 13 (depends on 1) The biological sheet culture device according to claim 1, further comprising: a liquid discharging assembly, wherein the liquid discharging assembly comprises the water permeable pipe that extends into the culture dish of the plurality of culture dishes from the top of the culture dish of the plurality of culture dishes and a negative pressure device outside the culture dish of the plurality of culture dishes and connected with the water permeable pipe, and wherein the negative pressure device is configured to discharge a culture medium out of the culture dish of the plurality of culture dishes through the water permeable pipe. ### Claim 14 (depends on 13) The biological sheet culture device according to claim 13, wherein the negative pressure device comprises a second pump. ### Claim 15 (depends on 1) The biological sheet culture device according to claim 1, wherein at least one culture dish of the plurality of culture dishes comprises a transparent material or a translucent material. ### Claim 16 (depends on 1) The biological sheet culture device according to claim 1, further comprising: an optical detection device over at least one culture dish of the plurality of culture dishes and configured to monitor a culture condition of at least one of the biological sheets in the at least one culture dish. ### Claim 17 (depends on 1) The biological sheet culture device according to claim 1, wherein the scaffold is associated with a culture dish height adjustor configured to adjust a height of at least one culture dish on the scaffold. ### Claim 18 A biological sheet culture facility comprising: an incubator; and at least one biological sheet culture, wherein the at least one biological sheet culture is detachable from the incubator, wherein each biological sheet culture device comprises: a scaffold; a plurality of culture dishes for culturing biological sheets positioned on the scaffold; and a plurality of communication pipes, each of which is configured to fluidly communicate with two adjacent culture dishes; wherein the plurality of culture dishes and the plurality of communication pipes are configured to form a plurality of fluid channels that are continuous, each fluid channel is configured to traverse every culture dish of the plurality of culture dishes, a sequence of one fluid channel of the plurality of fluid channels flowing through the plurality of culture dishes is different from that of at least one other fluid channel of the plurality of fluid channels flowing through the plurality of culture dishes wherein the plurality of fluid channels intersect each other through part of the plurality of culture dishes, wherein the plurality of fluid channels are configured such that adding culture medium to any single culture dish of the plurality of fluid channels can cause the culture medium to flow into all other culture dishes of the plurality of fluid channels, wherein an air permeable pipe is provided in a culture dish of the plurality of culture dishes, the air permeable pipe is configured to communicate from inside of the culture dish to outside of the culture dish, a permeable film is provided in the permeable pipe and configured to prevent dust and impurities from entering the culture dish through the air permeable pipe and allow air to enter the culture dish, and wherein the biological sheet culture device further comprises a water permeable pipe that extends into a culture dish of the plurality of culture dishes from a top of the culture dish of the plurality of culture dishes, and a fiber film is provided in the water permeable pipe and configured to prevent substances other than the culture medium in the culture dish from being discharged out of the culture dish through the water permeable pipe.