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US 11,999,928 B2Granted
Brewing method
AssigneeNovozymes A/S
InventorsAnne Mette Bhatia Frederiksen, Lars Beier, Stefan Kreisz
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Abstract
A method of mashing comprising providing a grist comprising malt and adjunct; and contacting the grist with a pullulanase; an alpha amylase; and a maltogenic alpha amylase and/or a beta amylase to make a wort. An enzyme composition comprising a pullulanase; an alpha amylase; and a maltogenic alpha amylase and/or a beta amylase and the use of these enzymes in brewing is disclosed.
Claims12
A method of mashing comprising:
- a) providing a grist comprising malt and adjunct, wherein the grist comprises 40-60% adjunct, wherein the adjunct has a gelatinization temperature higher than the malt starch, and wherein the adjunct is not gelatinized prior to mashing;
- and
- b) contacting the grist with the following combination of the enzymes, consisting of:
- i) a pullulanase;
- ii) an alpha amylase;
- and
- iii) a maltogenic alpha amylase, wherein the maltogenic alpha amylase is at least 10% more sucrose tolerant than a maltogenic alpha amylase comprising SEQ ID NO:
- 1, wherein the sucrose tolerance is measured in a buffer containing 10% sucrose (% w/v) at pH 5.0 for 15 minutes at 60° C. in accordance with example 5, to make a wort.
5 more elements
Dependencies are inferred from the claim text.