# US 11,999,780 B2 · Humanized anti-complement factor Bb antibodies and uses thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,999,780 B2 - Document ID: US11999780B2 - Status: Granted - Kind: B2 - Application number: 17/557,318 - Filed: 2021-12-21 - Published: 2022-07-07 - Granted: 2024-06-04 - Earliest claimed: 2020-04-20 - Assignees: Genzyme Corporation - Applicants: Genzyme Corporation - Inventors: Graham Parry; Stephen Moore; Michael Storek; Nina C. Leksa - Web page: https://patentgrep.com/patent/US11999780B2 ## CPC - C07K 16/18: against material from animals or humans - A61K 47/6843: {the antibody targeting a material from animals or humans} - C07K 2317/24: containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered - C07K 2317/31: multispecific - C07K 2317/33: Crossreactivity, e.g. for species or epitope, or lack of said crossreactivity - C07K 2317/52: Constant or Fc region; Isotype - C07K 2317/76: Antagonist effect on antigen, e.g. neutralization or inhibition of binding - C07K 2317/92: Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) ## Abstract Provided herein are humanized anti-factor Bb antibodies, methods of producing the antibodies and methods of using the antibodies. ## Claims ### Claim 1 A nucleic acid or nucleic acid set encoding a humanized antibody that binds specifically to human complement factor Bb protein, wherein the humanized antibody comprises: a heavy chain variable region (VH) comprising the amino acid sequence of SEQ ID NO: 19 and a light chain variable region (VL) comprising the amino acid sequence of SEQ ID NO: 27. ### Claim 2 (depends on 1) A vector or vector set comprising the nucleic acid or nucleic acid set of claim 1. ### Claim 3 (depends on 1) An isolated cell comprising the nucleic acid or nucleic acid set of claim 1. ### Claim 4 (depends on 3) The isolated cell of claim 3, wherein the cell is a mammalian cell. ### Claim 5 (depends on 4) The isolated cell of claim 4, wherein the mammalian cell is selected from human embryonic kidney (HEK) cells, Chinese hamster ovary (CHO) cells, NS0 myeloma cells, SP2 cells, COS cells, and mammary epithelial cells. ### Claim 6 (depends on 1) The nucleic acid or nucleic acid set of claim 1, wherein the humanized antibody comprises a heavy chain constant region comprising the amino acid sequence of any one of SEQ ID NOsi[H] 28-30. ### Claim 7 (depends on 1) The nucleic acid or nucleic acid set of claim 1, wherein the humanized antibody comprises a light chain constant region comprising the amino acid sequence of SEQ ID NO: 31. ### Claim 8 (depends on 6) The nucleic acid or nucleic acid set of claim 6, wherein the humanized antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 32 and a light chain comprising the amino acid sequence of SEQ ID NO: 35. ### Claim 9 (depends on 6) The nucleic acid or nucleic acid set of claim 6, wherein the humanized antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 33 and a light chain comprising the amino acid sequence of SEQ ID NO: 35. ### Claim 10 (depends on 6) The nucleic acid or nucleic acid set of claim 6, wherein the humanized antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 34 and a light chain comprising the amino acid sequence of SEQ ID NO: 35. ### Claim 11 (depends on 1) The nucleic acid or nucleic acid set of claim 1, wherein the humanized antibody is selected from the group consisting of an Ig monomer, a Fab fragment, a F(ab′)2 fragment, a scFv, a scAb, and a Fv. ### Claim 12 (depends on 1) The nucleic acid or nucleic acid set of claim 1, wherein the humanized antibody comprises a heavy chain constant region of the isotype IgG1, IgG2, IgG3, or IgG4. ### Claim 13 (depends on 3) A method of producing a humanized antibody that binds specifically to human complement factor Bb protein, the method comprising culturing the isolated cell of claim 3 to produce the humanized antibody. ### Claim 14 (depends on 13) The method of claim 13, further comprising isolating the humanized antibody. ### Claim 15 A composition comprising: a nucleic acid or nucleic acid set encoding a humanized antibody that binds specifically to human complement factor Bb protein, wherein the humanized antibody comprises: a heavy chain variable region (VH) comprising the amino acid sequence of SEQ ID NO: 19 and a light chain variable region (VL) comprising the amino acid sequence of SEQ ID NO: 27, and a buffer. ### Claim 16 A composition comprising: a vector or vector set comprising a nucleic acid or nucleic acid set encoding a humanized antibody that binds specifically to human complement factor Bb protein, wherein the humanized antibody comprises: a heavy chain variable region (VH) comprising the amino acid sequence of SEQ ID NO: 19 and a light chain variable region (VL) comprising the amino acid sequence of SEQ ID NO: 27, and a buffer. ### Claim 17 A composition comprising: an isolated cell comprising a nucleic acid or nucleic acid set encoding a humanized antibody that binds specifically to human complement factor Bb protein, wherein the humanized antibody comprises: a heavy chain variable region (VH) comprising the amino acid sequence of SEQ ID NO: 19 and a light chain variable region (VL) comprising the amino acid sequence of SEQ ID NO: 27, and a buffer.