Modified S1 subunit of the coronavirus spike protein
AssigneeBoehringer Ingelheim Vetmedia GmbH
InventorsAnnika Kraemer-Kuehl, Thomas Min Stephan, Hans-Christian Philipp
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Abstract
The present invention relates i.a. to a recombinant avian coronavirus spike protein or fragment thereof comprising a mutation at amino acid position 267 to Cysteine. Further, the present invention relates to an immunogenic composition comprising an avian coronavirus with such spike protein.
Claims18
A method for the production or manufacture of an avian coronavirus having extended cell or tissue tropism comprising mutating amino acid position 267 of an avian coronavirus spike protein or fragment of an avian coronavirus having restricted cell or tissue tropism to Cysteine, wherein the amino acid sequence of SEQ ID NO:1 is used for determining the position numbering in the spike protein, and wherein the mutation at amino acid position 267 to Cysteine leads to extended cell or tissue tropism of the avian coronavirus.
A method for the production or manufacture of an avian coronavirus having extended cell or tissue tropism comprising mutating amino acid position 267 of an avian coronavirus spike protein or fragment, wherein at least a part of the 51 subunit is from an avian coronavirus having restricted cell or tissue tropism, to Cysteine, wherein the amino acid sequence of SEQ ID NO:1 is used for determining the position numbering in the spike protein, and wherein the mutation at amino acid position 267 to Cysteine leads to extended cell or tissue tropism of the avian coronavirus.
A method for mutating the amino acid position 267 in an avian coronavirus spike protein comprising:
- a) providing an avian coronavirus or an avian coronavirus spike nucleotide or protein sequence,
- b) identifying position 267 in the spike protein by alignment with a reference sequence,
- c) mutating the position 267 of the spike protein of step b) into a cysteine,
- d) obtaining the mutated spike protein of step c).
Dependencies are inferred from the claim text.