# US 11,998,964 B2 · Methods and compositions for in situ groundwater remediation > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,998,964 B2 - Document ID: US11998964B2 - Status: Granted - Kind: B2 - Application number: 17/388,966 - Filed: 2021-07-29 - Published: 2021-11-18 - Granted: 2024-06-04 - Earliest claimed: 2017-05-24 - Applicants: Mork Technologies, LLC - Inventors: Benjamin V. Mork - Web page: https://patentgrep.com/patent/US11998964B2 ## CPC - B09C 1/002: {involving in-situ ground water treatment} - B09C 1/08: chemically - B09C 1/10: microbiologically, {biologically} or by using enzymes - C02F 1/68: by addition of specified substances, e.g. trace elements, for ameliorating potable water (medicinal water A61K) - C02F 3/342: {characterised by the enzymes used} - B09C 2101/00: In situ - C02F 1/283: {using coal, charred products, or inorganic mixtures containing them} - C02F 2103/06: Contaminated groundwater or leachate - B09C: RECLAMATION OF CONTAMINATED SOIL (gatherers for removing stone or the like from the soil A01B43/00; sterilising soil by steam A01G11/00; removing undesirable matter, e.g. rubbish, from the land E01H15/00) - C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes) ## Abstract Groundwater, slurry, or soil treatment or remediation compositions and methods of both production and use are described that include: at least one colloidal remediation chemical, at least one polysaccharide stabilizer, and at least one enzyme. ## Claims ### Claim 1 A groundwater treatment or remediation composition, comprising: at least one colloidal remediation chemical, at least one polysaccharide stabilizer, and at least one polysaccharide-degrading enzyme that does not interact with the untreated groundwater, the untreated soil, or a combination thereof. ### Claim 2 (depends on 1) The groundwater treatment or remediation composition of claim 1, further comprising water. ### Claim 3 (depends on 1) The groundwater treatment or remediation composition of claim 1, wherein the at least one colloidal remediation chemical comprises about 0.01 to about 50 weight percent of the composition. ### Claim 4 (depends on 2) The groundwater treatment or remediation composition of claim 2, wherein the at least one colloidal remediation chemical comprises about 0.01 to about 5 weight percent of the composition. ### Claim 5 (depends on 1) The groundwater treatment or remediation composition of claim 1, wherein the at least one polysaccharide stabilizer comprises about 0.01 to about 20 weight percent of the composition. ### Claim 6 (depends on 4) The groundwater treatment or remediation composition of claim 4, wherein the at least one polysaccharide stabilizer comprises about 0.01 to about 5 weight percent of the composition. ### Claim 7 (depends on 1) The groundwater treatment or remediation composition of claim 1, wherein the at least one enzyme comprises about 0.001 to about 5 weight percent of the composition. ### Claim 8 (depends on 6) The groundwater treatment or remediation composition of claim 6, wherein the at least one enzyme comprises about 0.001 to about 2 weight percent of the composition. ### Claim 9 (depends on 2) The groundwater treatment or remediation composition of claim 2, wherein the water comprises about 50 to about 99 weight percent of the composition. ### Claim 10 (depends on 9) The groundwater treatment or remediation composition of claim 9, wherein the water comprises about 95 to about 99 weight percent of the composition. ### Claim 11 (depends on 1) The groundwater treatment or remediation composition of claim 1, wherein the at least one colloidal remediation chemical comprises at least one reducing agent, at least one oxidant, at least one electron donor, at least one electron acceptor, at least one catalyst, at least one sorbent material, at least one metal-binding agent, at least one microbial culture, or a combination thereof. ### Claim 12 (depends on 1) The groundwater treatment or remediation composition of claim 1, wherein the at least one enzyme comprises a cellulase enzyme. ### Claim 13 A method of producing a groundwater treatment or remediation composition, comprising: providing at least one colloidal remediation chemical, providing at least one polysaccharide stabilizer, providing at least one polysaccharide-degrading enzyme, combining the at least one colloidal remediation chemical and the at least one polysaccharide stabilizer to form a treatment precursor, depositing the treatment precursor into a water source to form a pretreatment mixture; and combining the pretreatment mixture with the at least one enzyme, wherein the enzyme actively embeds and immobilizes at least part of the pretreatment mixture in at least one zone of untreated groundwater or untreated soil, at least one zone of contaminated groundwater or contaminated soil, or a combination thereof, without interacting with the untreated groundwater, untreated soil, or combination thereof. ### Claim 14 (depends on 13) The method of claim 13, wherein the at least one enzyme comprises a cellulase enzyme. ### Claim 15 A method of producing a groundwater treatment or remediation composition, comprising: providing at least one colloidal remediation chemical, providing at least one polysaccharide stabilizer, providing at least one polysaccharide-degrading enzyme, wherein the enzyme actively embeds and immobilizes at least part of the treatment or remediation composition in at least one zone of untreated groundwater or untreated soil, at least one zone of contaminated groundwater or contaminated soil, or a combination thereof, without interacting with the untreated groundwater, untreated soil, or combination thereof, and combining the at least one colloidal remediation chemical, the at least one polysaccharide stabilizer, and the at least one enzyme to form a treatment composition. ### Claim 16 (depends on 15) The method of claim 15, wherein the at least one enzyme comprises a cellulase enzyme.