# US 11,998,917 B2 · Microfluidic device and a method of loading fluid therein > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,998,917 B2 - Document ID: US11998917B2 - Status: Granted - Kind: B2 - Application number: 17/167,228 - Filed: 2021-02-04 - Published: 2021-06-17 - Granted: 2024-06-04 - Earliest claimed: 2015-09-16 - Assignees: Sharp Life Science (EU) Limited - Applicants: Sharp Life Science (EU) Limited - Inventors: Emma Jayne Walton; Lesley Anne Parry-Jones; Julie Karen Deacon - Web page: https://patentgrep.com/patent/US11998917B2 ## CPC - B01L 3/502792: {for moving individual droplets on a plate, e.g. by locally altering surface tension} - B01L 3/502715: {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces} - B01L 3/502723: {characterised by venting arrangements} - B01L 2200/027: for microfluidic devices - B01L 2200/0605: Metering of fluids - B01L 2200/0642: Filling fluids into wells by specific techniques - B01L 2200/0673: Handling of plugs of fluid surrounded by immiscible fluid - B01L 2200/0684: Venting, avoiding backpressure, avoid gas bubbles - B01L 2300/0809: rectangular shaped - B01L 2300/0816: Cards, e.g. flat sample carriers usually with flow in two horizontal directions - B01L 2300/0864: comprising only one inlet and multiple receiving wells, e.g. for separation, splitting - B01L 2300/165: Specific details about hydrophobic, oleophobic surfaces - B01L 2400/0406: capillary forces - B01L 2400/0427: Electrowetting - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE ## Abstract A microfluidic AM-EWOD device and a method of filling such a device are provided. The device comprises a chamber having one or more inlet ports. The device is configured, when the chamber contains a metered volume of a filler fluid that partially fills the chamber, preferentially maintain the metered volume of the filler fluid in a part of the chamber. The device is configured to allow displacement of some of the filler fluid from the part of the chamber when a volume of an assay fluid introduced into one of the one or more inlet ports enters the part of the chamber, thereby causing a volume of a venting fluid to vent from the chamber. ## Claims ### Claim 1 A method of loading an electro-wetting on dielectric (EWOD) device with an assay fluid comprising: providing the EWOD device, wherein the EWOD device comprises: first and second substrates spaced from one another by a spacer and a fluid barrier, the EWOD device further including side walls and the first substrate being an upper substrate and the second substrate being a lower substrate such that the first and second substrates and the side walls define a chamber therebetween; and a plurality of ports that permit introduction of fluid into the chamber and/or displacement of fluid from the chamber; the chamber including a vent area containing a venting fluid and an active area for carrying out an assay, and the plurality of ports includes at least one input port, a first vent port located in the vent area, and a second vent port located in the active area; and the method further comprising: introducing a filler fluid into the active area of the chamber through one or more of the plurality of ports, filling the active area of the chamber with the filler fluid; and introducing a volume of the assay fluid into the chamber through the at least one input port, wherein a portion of the venting fluid vents through the first vent port and a portion of the filler fluid is displaced into the second vent port upon entry of the volume of the assay fluid into the chamber. ### Claim 2 (depends on 1) The method as claimed in claim 1, wherein the EWOD device comprises electrodes, and the method further comprises controlling the assay fluid within the chamber by actuation of said electrodes. ### Claim 3 (depends on 2) The method as claimed in claim 2, and comprising controlling the assay fluid within the chamber by actuation of said electrodes during the introduction of the assay fluid into the active area of the chamber. ### Claim 4 (depends on 1) The method as claimed in claim 1, wherein the volume of the assay fluid is introduced into the active area of chamber after the filler fluid has been introduced into the active area of the chamber, whereby the assay fluid enters the active area of the chamber by displacing some of the filler fluid from the active area of the chamber. ### Claim 5 (depends on 1) The method as claimed in claim 1, wherein at least part of an interior of the chamber is coated with a hydrophobic coating. ### Claim 6 (depends on 1) The method as claimed in claim 1, wherein the one or more of the plurality of ports are provided in an upper surface of the chamber or in one or more sides of the chamber. ### Claim 7 (depends on 1) The method as claimed in claim 1, further comprising introducing a second assay fluid via another one or more of the plurality of ports. ### Claim 8 (depends on 1) The method as claimed in claim 1, wherein the filler fluid comprises a non-polar fluid, an oil, or a surfactant. ### Claim 9 (depends on 1) The method as claimed in claim 1, wherein the assay fluid comprises a polar fluid, an aqueous material, or a surfactant. ### Claim 10 A microfluidic device, comprising: first and second substrates spaced from one another by a spacer and a fluid barrier, and side walls, the first substrate being an upper substrate and the second substrate being a lower substrate such that the first and second substrates and the side walls define a chamber therebetween; and a plurality of ports that permit introduction of fluid into the chamber and/or displacement of fluid from the chamber; the chamber including a vent area containing a venting fluid and an active area, and the plurality of ports includes at least one input port, a first vent port located in the vent area, and a second vent port located in the active area; wherein the active area of the chamber is configured to receive a filler fluid that is introduced into the active area of the chamber through one or more of the plurality of ports filling the active area of the chamber with the filler fluid, and the active area of the chamber is configured to receive a volume of an assay fluid introduced into the active area of the chamber through the at least one input port, and wherein the first vent port is positioned such that a portion of the venting fluid vents through the first vent port, and the second vent port is positioned such that a portion of the filler fluid is displaced into the second vent port, upon entry of the volume of the assay fluid into the chamber. ### Claim 11 (depends on 10) The microfluidic device as claimed in claim 10, further comprising electrodes for controlling the assay fluid within the chamber by actuation of said electrodes. ### Claim 12 (depends on 10) The microfluidic device as claimed in claim 10, wherein at least part of an interior of the chamber is coated with a hydrophobic coating. ### Claim 13 (depends on 10) The microfluidic device as claimed in claim 10, wherein one or more of the plurality of ports are provided in an upper surface of the chamber or in one or more sides of the chamber. ### Claim 14 (depends on 10) A microfluidic system comprising a microfluidic device as claimed in claim 10, said device contained within a disposable cartridge, and a reader device configured to control the microfluidic device.