Back to results
US 11,998,907 B2Granted
Cartridges, systems and methods for mass spectrometry
AssigneeThe Trustees of Indiana University
InventorsNicholas Edward Manicke, Chengsen Zhang
Life of this patent
Estimating the term…
Abstract
The present disclosure provides “all-in-one” cartridges which contain necessary reagents and materials to isolate/preconcentrate targeted proteins from blood plasma and ionize them for mass spectrometry detection. In another configuration, the cartridges include proteolytic enzymes to digest the proteins into smaller peptides in addition to preconcentration and ionization for mass spectrometry detection.
Claims20
A protein detection cartridge comprising:
- a lid including an opening;
- a base coupled to the lid and including a recess;
- a column holder removably positioned in the opening, the column holder including an aperture;
- an antibody column received within the aperture and moveable within the aperture;
- a waste pad removably positioned in the recess of the base;
- a spray tip holder positioned in the base, the spray tip holder including a recess;
- and
- an enzyme immobilized membrane positioned in the recess of the spray tip holder;
- wherein the column holder is movable between a first position where the antibody column is positioned over the waste pad and a second position where the antibody column is positioned over the enzyme immobilized membrane;
- and
- wherein, with the column holder in the second position, the antibody column is configured to be pushed downwardly into the recess of the spray tip holder and into contact with the enzyme immobilized membrane.
8 more elements
A protein detection cartridge comprising:
- a lid including an opening;
- a base coupled to the lid and including a recess;
- a column holder removably positioned in the opening;
- the column holder including an aperture;
- an antibody column received within the aperture and moveable within the aperture;
- a waste pad removably positioned in the recess of the base;
- a spray tip holder positioned in the base, the spray tip holder including a recess;
- an enzyme immobilized membrane positioned in the recess of the spray tip holder;
- and
- a spray substrate coupled to the spray tip holder such that the enzyme immobilized membrane is positioned over the spray substrate;
- wherein the column holder is slidable within the cartridge between a first position and a second position;
- and
- wherein, in the first position, the antibody column is positioned over the waste pad and, in the second position, the antibody column is positioned over the enzyme immobilized membrane and the spray substrate;
- and
- wherein, in the second position, the antibody column is moveable, within the aperture, into the recess of the spray tip holder and into contact with the enzyme immobilized membrane.
12 more elements
A method of using a protein detection cartridge for mass spectrometry comprising:
- setting a column holder of the protein detection cartridge in a first position, wherein the column holder includes an aperture movably receiving an antibody column therein;
- inserting a plasma sample into the antibody column of the protein detection cartridge, the plasma sample comprising proteins;
- with the column holder in the first position, inserting a wash buffer into the antibody column;
- sliding the column holder to a second position, wherein the antibody column is above an enzyme immobilized membrane positioned in a recess of the protein detection cartridge;
- pushing the antibody column into the recess and into contact with the enzyme immobilized membrane;
- and
- inserting an elution buffer into the antibody column to cause the proteins in the antibody column to exit onto the enzyme immobilized membrane, the enzyme immobilized membrane digesting the proteins to form a peptide.
4 more elements
Dependencies are inferred from the claim text.