# US 11,998,479 B2 · Method and system for addressing adverse effects on the oral microbiome and restoring gingival health caused by sodium lauryl sulphate exposure > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,998,479 B2 - Document ID: US11998479B2 - Status: Granted - Kind: B2 - Application number: 18/234,544 - Filed: 2023-08-16 - Published: 2024-01-25 - Granted: 2024-06-04 - Earliest claimed: 2011-02-04 - Assignees: Seed Health, Inc. - Applicants: Seed Health, Inc. - Inventors: Sheri Simmons; Tye Jensen; Joseph E. Kovarik - Web page: https://patentgrep.com/patent/US11998479B2 ## CPC - A61F 5/566: {Intra-oral devices} - A61Q 11/00: Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses - A61C 5/007: {Dental splints; teeth or jaw immobilisation devices; stabilizing retainers bonded to teeth after orthodontic treatments} - A61K 8/27: Zinc; Compounds thereof - A61K 8/498: {having 6-membered rings or their condensed derivatives, e.g. coumarin} - A61K 8/99: from microorganisms other than algae or fungi, e.g. protozoa or bacteria - A61P 1/02: Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis - A61F: FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS (dental prosthetics A61C) - A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS - A61C: DENTISTRY; APPARATUS OR METHODS FOR ORAL OR DENTAL HYGIENE (non-driven toothbrushes A46B; {tongue scrapers A61B17/24;} preparations for dentistry A61K6/00; preparations for cleaning the teeth or mouth A61K8/00, A61Q11/00) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS ## Abstract A method and system to address the adverse effects on an individual's oral microbiome with the objective of restoring gingival health caused by exposure to sodium lauryl sulfate. Certain embodiments of the present invention facilitate the growth of desired bacteria in a human's mouth to reduce the likelihood of dental caries, halitosis, canker sores, and other oral diseases. Probiotic bacterial, bioactive flavonoid, xylitol and zinc compound compositions, provide effective prevention and treatment options, especially while precluding the use of sodium lauryl sulfate so as to reduce adverse consequences it has on gingival health. ## Claims ### Claim 1 A method for reducing the likelihood of developing dental caries and halitosis, comprising: providing to an oral cavity of an individual subject: a first bacterial composition comprising beneficial bacteria adapted to form a biofilm, said beneficial bacteria comprising a bacterium of the genus Rothia; a second composition comprising: an oral probiotic comprising at least two of the following bacteria: Lactobacillus brevis CD2, Streptococcus sanguinis BCC23, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus johnsonii, Bifidobacterium bifidum, Enterococcus faecium, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus paracasei, Lactobacillus casei, Lachnospira, Veillonella, Faecalibacterium and Prevotella; at least one zinc-based compound selected from the group consisting of zinc acetate, zinc chloride, zinc citrate, and zinc sulphate; and at least one bioactive flavonoid selected from the group consisting of baicalein, naringenin, catechins, quercetin, trans-resveratrol, luteolin, kaempferol, hesperidin, hesperetin, naringin, diosmin, rutin, nobiletin, and tangeretin; and wherein the oral probiotic lowers the pH of the individual subject's oral cavity in an amount sufficient to disrupt the formation of biofilms by pathogenic bacteria. ### Claim 2 (depends on 1) The method of claim 1, wherein the step of providing comprises topically administering said second composition in the form of one of a toothpaste, mouth wash, chewing gum, oral strip, extruded chew and jerky chew. ### Claim 3 (depends on 1) The method of claim 1, further comprising, using a clustered regularly interspaced short palindromic repeats (CRIPSR)-CRISPR associated protein (Cas) system or a CRISPR from Prevotella and Franscisella 1 (Cpf1a) system, selectively killing pathogenic bacteria in the individual subject's oral cavity. ### Claim 4 (depends on 1) The method of claim 1, wherein said step of providing the first bacterial composition is performed after the individual subject has cleaned their teeth with a toothpaste devoid of sodium lauryl sulphate (SLS). ### Claim 5 (depends on 1) The method of claim 1, wherein said second composition comprises Veillonella bacteria. ### Claim 6 (depends on 1) The method of claim 1, wherein said second composition further comprises breath freshening components. ### Claim 7 (depends on 1) The method as set forth in claim 1, further comprising providing at least one bacteria that has been modified by employment of a CRISPR-Cas or Cpf1 system to remove a virulence factor selected from the group consisting of gelatinase and hemolysin. ### Claim 8 (depends on 1) The method as set forth in claim 1, wherein the individual subject is a human. ### Claim 9 (depends on 1) The method as set forth in claim 1, wherein the individual subject is a canine. ### Claim 10 (depends on 1) The method as set forth in claim 1, wherein the first bacterial composition and the second are combined. ### Claim 11 A method for reducing the likelihood of developing dental caries and halitosis, comprising: providing to an oral cavity of an individual subject: a first bacterial composition comprising beneficial bacteria adapted to form a biofilm, said beneficial bacteria comprising a bacterium of the genus Rothia, and wherein said bacterial composition does not inhibit growth of a bacterium of species Lactobacillus salivarius; said step of providing performed after the individual subject has cleaned their teeth with a toothpaste devoid of sodium lauryl sulphate (SLS); a second composition comprising: an oral probiotic comprising at least two of the following bacteria: Lactobacillus brevis CD2, Streptococcus sanguinis BCC23, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus johnsonii, Bifidobacterium bifidum, Enterococcus faecium, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus paracasei, Lactobacillus casei, Lachnospira, Veillonella, Faecalibacterium and Prevotella; at least one zinc-based compound selected from the group consisting of zinc acetate, zinc chloride, zinc citrate, and zinc sulphate; and at least one bioactive flavonoid selected from the group consisting of baicalein, naringenin, catechins, quercetin, trans-resveratrol, Luteolin, kaempferol, hesperidin, hesperetin, naringin, diosmin, rutin, nobiletin, and tangeretin; and wherein the halitosis is caused by a pathogenic bacteria that generates volatile sulfur compounds comprising one of methylmercaptan and hydrogen sulfite. ### Claim 12 (depends on 11) The method as set forth in claim 11, further comprising using a clustered regularly interspaced short palindromic repeats (CRIPSR)-CRISPR associated protein (Cas) system or a CRISPR from Prevotella and Franscisella 1 (Cpf1a) system, selectively killing pathogenic bacteria in the individual subject's oral cavity. ### Claim 13 (depends on 11) The method as set forth in claim 11, wherein said pathogenic bacteria is Porphyromonas gingivalis. ### Claim 14 (depends on 11) The method as set forth in claim 11, wherein the individual subject is a human. ### Claim 15 (depends on 14) The method as set forth in claim 14, wherein the individual subject is a canine. ### Claim 16 A method for reducing the likelihood of developing dental caries and halitosis, comprising: providing to an oral cavity of an individual subject: a first bacterial composition comprising beneficial bacteria adapted to form a biofilm, said beneficial bacteria comprising a bacterium of the genus Rothia, and wherein said bacterial composition does not inhibit growth of a bacterium of species Lactobacillus salivarius; said step of providing performed after the individual subject has cleaned their teeth; a second composition comprising: an oral probiotic comprising at least two of the following bacteria: Lactobacillus brevis CD2, Streptococcus sanguinis BCC23, Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus casei strain Shirota, and Veillonella; at least one zinc-based compound selected from the group consisting of zinc acetate, zinc chloride, zinc citrate, and zinc sulphate; and at least one bioactive flavonoid selected from the group consisting of quercetin, nobiletin, tangeretin; and xylitol; and wherein said step of providing the first bacterial composition is performed after the individual subject has cleaned their teeth with a toothpaste devoid of sodium lauryl sulphate (SLS). ### Claim 17 (depends on 1) The method of claim 1, wherein said second composition is in the form of one of a toothpaste, mouth wash, gargle solution, nose spray, mouth spray, throat spray, chewing gum, hydrogel, oral strip, and oral cream. ### Claim 18 (depends on 1) The method of claim 1, wherein said oral probiotic comprises Lactobacillus casei, strain Shirota. ### Claim 19 (depends on 16) The method as set forth in claim 16, further comprising providing at least one bacteria that has been modified by employment of a CRISPR-Cas or Cpf1 system to remove a virulence factor selected from the group consisting of gelatinase and hemolysin. ### Claim 20 (depends on 16) The method of claim 16, further comprising, using a clustered regularly interspaced short palindromic repeats (CRIPSR)-CRISPR associated protein (Cas) system or a CRISPR from Prevotella and Franscisella 1 (Cpf1a) system, selectively killing pathogenic bacteria in the individual subject's oral cavity.