# US 11,998,016 B2 · Use of Chaetomium globosum strain in controlling fusarium crown rot of wheat

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- Number: US 11,998,016 B2
- Document ID: US11998016B2
- Status: Granted
- Kind: B2
- Application number: 17/233,732
- Filed: 2021-04-19
- Published: 2022-02-24
- Granted: 2024-06-04
- Earliest claimed: 2020-08-18
- Assignees: Institute of Plant Protection, Henan Academy of Agricultural Sciences
- Applicants: Institute of Plant Protection, Henan Academy of Agricultural Sciences
- Inventors: Fei Xu; Yuli Song; Junmei Wang; Jiaojiao Zhang; Zixing Han; Yahong Li; Ruijie Shi; Lijuan Li; Lulu Liu
- Web page: https://patentgrep.com/patent/US11998016B2

## CPC

- A01N 63/30: Microbial fungi; Substances produced thereby or obtained therefrom
- A01N 25/04: Dispersions, {emulsions, suspoemulsions, suspension concentrates} or gels (foams A01N25/16)
- A01N 65/00: Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof (containing compounds of determined constitution A01N27/00 - A01N59/00)
- A01P 3/00: Fungicides
- C12N 15/00: Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor (mutants or genetically engineered microorganisms, per se C12N1/00, C12N5/00, C12N7/00; new plants per se A01H; plant reproduction by tissue culture techniques A01H4/00; new animals per se A01K67/00; use of medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases, gene therapy A61K48/00)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01P: BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present disclosure provides use of a Chaetomium globosum strain in controlling Fusarium crown rot (FCR) of wheat. The C. globosum strain under the deposit designation of 12XP1-2-3 is deposited at China General Microbiological Culture Collection Center (CGMCC) on Jan. 23, 2019 with an accession number of CGMCC No. 17183. Field experiments conclude that coating of wheat seeds with Chaetomium globosum strain 12XP1-2-3 can reduce diseased stem rate, disease rating, and disease index by 19.0%-41.3%, 43.7%, and 4.7%-45.4%, respectively.

## Claims

### Claim 1

A method for controlling Fusarium crown rot (FCR) of wheat, the method comprising applying a Chaetomium globosum strain or a biocontrol agent containing the Chaetomium globosum strain to a FCR fungus,
wherein the Chaetomium globosum strain is strain 12XP1-2-3 deposited at China General Microbiological Culture Collection Center (CGMCC) on Jan. 23, 2019 with an accession number of CGMCC No. 17183;
wherein strain 12XP1-2-3 is identified by PCR using sequences ITS1 (5′-TCC GTA GGT GAA CCT GCG G-3′) and ITS4 (5′-TCC TCC GCT TAT TGA TAT GC-3′) as primers for amplification of DNA fragments containing ITS1, 5.8S rDNA and ITS2 in strain 12XP1-2-3; and
wherein wheat seeds are coated at a coating concentration of 5.0-5.3×104 ascospores per seed.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the biocontrol agent is prepared by multiplying the Chaetomium globosum strain to obtain a spore suspension, and dispersing the spore suspension in a sodium carboxymethyl cellulose solution to obtain the biocontrol agent.

### Claim 3 (depends on 2)

The method according to claim 2, wherein the sodium carboxymethyl cellulose solution has a mass fraction of 4%, and the spore suspension and the sodium carboxymethyl cellulose solution have a volume ratio of 3:1.