# US 11,998,009 B2 · Biostimulant concentrate and methods thereof

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- Number: US 11,998,009 B2
- Document ID: US11998009B2
- Status: Granted
- Kind: B2
- Application number: 17/164,434
- Filed: 2021-02-01
- Published: 2021-08-19
- Granted: 2024-06-04
- Earliest claimed: 2020-01-31
- Assignees: AG Biotech, LLC
- Applicants: AG Biotech, Inc.
- Inventors: Robert M. Hudak; Tristan C. Hudak
- Web page: https://patentgrep.com/patent/US11998009B2

## CPC

- A01N 43/22: rings with more than six members
- A01N 25/04: Dispersions, {emulsions, suspoemulsions, suspension concentrates} or gels (foams A01N25/16)
- A01N 25/22: containing ingredients stabilising the active ingredients
- A01N 25/30: characterised by the surfactants
- A01N 31/02: Acyclic compounds
- A01P 17/00: Pest repellants
- A01P 21/00: Plant growth regulators
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01P: BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS

## Abstract

A biostimulant concentrate for direct application to agricultural crops comprising purified water and a solubilizer, triacontanol in a concentration of between 500-4000 milligrams per liter (mg/l), brassinolide in a concentration of between 30-120 milligrams per liter (mg/l), and a stabilizer is selected from the group consisting of nano silver, nano copper, and combinations thereof.

## Claims

### Claim 1

A biostimulant concentrate in liquid form comprising:
purified water;
a solubilizer, wherein the solubilizer is polysorbate 80 and is present in the biostimulant concentrate in a concentration of between 3-7 g/l;
triacontanol; and
brassinolide;
wherein the weight ratio of brassinolide to triacontanol is between 1:50 to 1:40.

### Claim 2 (depends on 1)

The biostimulant concentrate as set forth in claim 1, wherein the pH of the biostimulant concentrate is 6.2.

### Claim 3 (depends on 1)

The biostimulant concentrate as set forth in claim 1, wherein the pH of the biostimulant concentrate is between 6.2-6.4.

### Claim 4

A method of preparing a biostimulant concentrate comprising the steps of:
providing and heating purified water to between 60-90° C.;
mixing a solubilizer with the purified water, wherein the solubilizer is polysorbate 80 and is mixed with the purified water in a concentration of between 3-7 g/l to obtain a solubilizer solution;
mixing triacontanol with the solubilizer solution to obtain a triacontanol solution;
cooling the triacontanol solution to room temperature, wherein the temperature is between 20-23.5° C.; and
mixing brassinolide with the triacontanol solution to obtain the biostimulant concentrate;
wherein the weight ratio of brassinolide to triacontanol is between 1:50 to 1:40.

### Claim 5 (depends on 4)

The method as set forth in claim 4, wherein the biostimulant concentrate comprises the solubilizer in a concentration of 5 g/l.

### Claim 6 (depends on 4)

The method as set forth in claim 4, wherein the biostimulant concentrate comprises the brassinolide in a concentration of approximately 60 mg/l.

### Claim 7 (depends on 4)

The method as set forth in claim 4, wherein the biostimulant concentrate comprises the triacontanol in a concentration of approximately 3000 mg/l.

### Claim 8 (depends on 4)

The method as set forth in claim 4, wherein the pH of the biostimulant concentrate is at least 6.2.

### Claim 9 (depends on 4)

The method as set forth in claim 4, wherein the pH of the biostimulant concentrate is between 6.2-6.4.

### Claim 10 (depends on 4)

The method as set forth in claim 4, wherein the biostimulant concentrate comprises the brassinolide in a concentration of approximately 60 mg/l and the triacontanol in a concentration of approximately 3000 mg/l, having a weight ratio of brassinolide to triacontanol of approximately 1:50.

### Claim 11 (depends on 4)

The method as set forth in claim 4, wherein the biostimulant concentrate comprises the brassinolide in a concentration of at least 50 mg/l and the triacontanol in a concentration of at least 2000 mg/l, having a weight ratio of brassinolide to triacontanol of approximately 1:40.