# US 11,994,471 B2 · Optical signal detection modules and methods > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,994,471 B2 - Document ID: US11994471B2 - Status: Granted - Kind: B2 - Application number: 18/159,638 - Filed: 2023-01-25 - Published: 2023-11-09 - Granted: 2024-05-28 - Earliest claimed: 2017-07-26 - Assignees: Gen-Probe Incorporated - Applicants: Gen-Probe Incorporated - Inventors: Norbert Hagen; George Walker; David Combs - Web page: https://patentgrep.com/patent/US11994471B2 ## CPC - G01N 21/6486: {Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence)} - C12Q 1/6806: Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) - G01N 21/6452: {Individual samples arranged in a regular 2D-array, e.g. multiwell plates} - G02B 6/08: with fibre bundle in form of plate - G01N 2021/6484: {Optical fibres} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS ## Abstract An apparatus for detecting an optical signal emission includes signal transmission fibers. Each fiber includes cores having the same spatial core arrangement at each end. The first ends are configured to be optically coupled to the signal emission sources. Each fiber is configured to transmit an optical signal between the first end and the second. The apparatus can also include a frame assembly securing the first ends of the fibers in a first spatial fiber arrangement corresponding to a spatial arrangement of the signal emission sources. The frame assembly can also secure the second ends of the fibers in a second spatial fiber arrangement different from the first spatial fiber arrangement. The apparatus can also include at least one signal detector configured to be optically coupled to the second ends of the fibers and configured to detect an optical signal emitted by each signal emission source. ## Claims ### Claim 1 A system for detecting an optical signal emission from at least one signal emission source, comprising: at least one signal transmission fiber comprising a plurality of cores having a first spatial core arrangement at a first end and a second spatial core arrangement at a second end that is the same as the first spatial core arrangement, the first end is configured to be optically coupled to a respective signal emission source such that an optical signal emitted by the respective signal emission source is transmitted from the first end of the at least one signal transmission fiber to the second of the at least one signal transmission fiber; and at least one signal detector configured to generate an excitation light and detect the optical signal emitted by the respective signal emission source, wherein the at least one signal detector is configured to be optically coupled to the second end of the at least one signal transmission fiber such that the excitation light is transmitted from the second end of the at least one signal transmission fiber to the first end of the at least one transmission fiber and the optical signal emitted by the respective signal emission source is detected at the second end of the signal transmission fiber, wherein the at least one signal detector and the second end of the at least one signal transmission fiber being arranged such that (i) an intensity pattern of the transmitted excitation light at the first end of the at least one signal transmission fiber that is symmetric is an indication that the at least one signal detector and the second end of the at least one signal transmission fiber are optically aligned, and (ii) an intensity pattern of the transmitted excitation light at the first end of the at least one signal transmission fiber that is asymmetric is an indication that the at least one signal detector and the second end of the at least one signal transmission fiber are optically misaligned. ### Claim 2 (depends on 1) The system of claim 1, wherein the optical signal emitted by the respective signal emission source is transmitted at a predetermined emission wavelength, and the at least one signal detector comprises an excitation source configured to generate the excitation light at a predetermined excitation wavelength that is different than the emission wavelength. ### Claim 3 (depends on 2) The system of claim 2, wherein the excitation source comprises at least one light-emitting-diode. ### Claim 4 (depends on 1) The system of claim 1, wherein the at least one signal detector comprises a photodiode to detect the optical signal emitted by the respective signal emission source. ### Claim 5 (depends on 1) The system of claim 1, wherein the at least one signal detector comprises a fluorometer. ### Claim 6 (depends on 1) The system of claim 1, wherein the at least one signal transmission fiber comprises a plurality of signal transmission fibers each comprising a plurality of cores having a first spatial core arrangement at a first end and a second spatial core arrangement at a second end that is the same as the first spatial core arrangement. ### Claim 7 (depends on 6) The system of claim 6, wherein: the at least one signal detector comprises a plurality of signal detectors, wherein each signal detector is configured to generate an excitation light of a different predetermined excitation wavelength and to detect an optical signal of a different predetermined emission wavelength. ### Claim 8 (depends on 7) The system of claim 7, further comprising: a signal detector carrier having mounted thereon the plurality of signal detectors, the signal detector carrier being configured to move such that each signal detector is sequentially and optically coupled to the second ends of the plurality of signal transmission fibers. ### Claim 9 (depends on 6) The system of claim 6, further comprising: a frame assembly securing the first ends of the plurality of signal transmission fibers in a first spatial fiber arrangement corresponding to a spatial arrangement of the signal emission sources, and securing the second ends of the plurality of signal transmission fibers in a second spatial fiber arrangement different from the first spatial fiber arrangement. ### Claim 10 (depends on 9) The system of claim 9, wherein the frame assembly comprises: an interface plate securing the first ends of the plurality of signal transmission fibers in the first spatial fiber arrangement; and a base, spaced apart from the interface plate, securing the second ends of the plurality of signal transmission fibers in the second spatial arrangement. ### Claim 11 (depends on 1) The system of claim 1, further comprising: a camera system configured to acquire an image of an intensity pattern of the excitation light at the first end of the at least one of signal transmission fiber. ### Claim 12 (depends on 11) The system of claim 11, wherein the system is configured to determine automatically whether the acquired image of the intensity pattern of the excitation light is symmetric or asymmetric. ### Claim 13 (depends on 11) The system of claim 11, further comprising a display device configured to display a magnified image of the intensity pattern of the excitation light at the first end of the at least one of signal transmission fiber. ### Claim 14 (depends on 1) The system of claim 1, wherein: each core of the plurality of cores comprises polymethyl methacrylate (PMMA); and each core of the plurality of cores is encased by a cladding comprising a fluorinated polymer. ### Claim 15 (depends on 1) The system of claim 1, wherein each core has a non-circular cross-sectional shape. ### Claim 16 (depends on 1) A method of diagnosing an optical misalignment between the at least one signal detector and the second end of the at least one signal transmission fiber of the system of claim 1, comprising: generating an excitation light from the at least one signal detector; transmitting the excitation light from the second end of the at least one signal transmission fiber to the first end of the at least one signal transmission fiber; determining whether an intensity pattern of the transmitted excitation light at the first end of the at least one signal transmission fiber is symmetric or asymmetric. ### Claim 17 (depends on 16) The method of claim 16, wherein the determining step is manually performed by visually inspecting the intensity pattern. ### Claim 18 (depends on 17) The method of claim 17, wherein the visually inspecting comprises using a magnifier that generates a magnified image of the intensity pattern. ### Claim 19 (depends on 16) The method of claim 16, wherein the determining step comprises: acquiring, by a camera system, an image of the intensity pattern at the first end of the at least one signal transmission fiber; and automatically analyzing, by the camera system, the acquired image to determine whether the intensity pattern of the transmitted excitation light is symmetric or asymmetric.