Pichinde virus reverse genetics systems and methods of use

A collection of vectors comprising:

• a first vector encoding a first genomic segment comprising a coding region encoding a Z protein and a coding region encoding a L RdRp protein, wherein the coding region encoding the Z protein is antigenomic,
• a second vector encoding a second genomic segment comprising a coding region encoding a nucleoprotein (NP), wherein the coding region encoding the NP is antigenomic, and
• a third vector encoding a third genomic segment comprises a coding region encoding a glycoprotein, wherein the coding region encoding the glycoprotein is antigenomic;
• and
• wherein the second genomic segment further comprises a coding region encoding a first protein, or the third genomic segment further comprises a coding region encoding a second protein.

A reverse genetics system for making a genetically engineered Pichinde virus comprising three vectors,

• wherein a first vector encodes a first genomic segment comprising a coding region encoding a Z protein and a coding region encoding a L RdRp protein, wherein the coding region encoding the Z protein is antigenomic,
• wherein the second vector encodes a second genomic segment comprising a coding region encoding a nucleoprotein (NP), wherein the coding region encoding the NP is antigenomic,
• wherein the third vector encodes a third genomic segment comprises a coding region encoding a glycoprotein, wherein the coding region encoding the glycoprotein is antigenomic
• wherein the second genomic segment further comprises a coding region encoding a first protein, or the third genomic segment further comprises a coding region encoding a second protein.