# US 11,993,762 B2 · Polypeptides having alpha-mannan degrading activity and polynucleotides encoding same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,993,762 B2 - Document ID: US11993762B2 - Status: Granted - Kind: B2 - Application number: 17/280,798 - Filed: 2019-10-02 - Published: 2022-03-24 - Granted: 2024-05-28 - Earliest claimed: 2018-10-03 - Assignees: Novozymes A/S - Applicants: Novozymes A/S - Inventors: Jesper Salomon; Klaus Gori; Lorena González Palmén; Kenneth Jensen; Nikolaj Spodsberg; Mary Ann Stringer; Kristian Bertel Roemer M. Krogh; Morten Gjermansen - Web page: https://patentgrep.com/patent/US11993762B2 ## CPC - C11D 3/38636: {containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase} - C12N 9/2402: {hydrolysing O- and S- glycosyl compounds (3.2.1)} - C12Y 302/01078: Mannan endo-1,4-beta-mannosidase (3.2.1.78), i.e. endo-beta-mannanase - C12N 15/09: Recombinant DNA-technology - C12N 15/11: DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity} - C12P 21/00: Preparation of peptides or proteins (single cell protein C12N1/00) - C12P 21/02: having a known sequence of two or more amino acids, e.g. glutathione - C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12Y: ENZYMES - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} ## Abstract The invention relates to compositions comprising and uses of polypeptides having alpha-mannan degrading activity. The present invention relates to polypeptides having alpha-mannan degrading activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. ## Claims ### Claim 1 A cleaning composition comprising: (a) at least one polypeptide having alpha-mannan degrading activity or a variant or a fragment thereof having alpha-mannan degrading activity, wherein the at least one polypeptide, the variant or the fragment thereof is selected from the group consisting of: (I) a polypeptide having at least 96% sequence identity but less than 100% sequence identity to SEQ ID NO: 2 or SEQ ID NO: 3 (mature form), or a polypeptide is encoded by a polynucleotide having at least 90% sequence identity but less than 100% sequence identity to SEQ ID NO: 1; (II) a polypeptide having at least 70% sequence identity but less than 100% sequence identity to SEQ ID NO: 5 or SEQ ID NO: 6 (mature form), or a polypeptide is encoded by a polynucleotide having at least 60% sequence identity but less than 100% sequence identity to SEQ ID NO: 4; (III) a polypeptide having at least 75% sequence identity but less than 100% sequence identity to SEQ ID NO: 8 or SEQ ID NO: 9 (mature form), or a polypeptide is encoded by a polynucleotide having at least 60% sequence identity but less than 100% sequence identity to SEQ ID NO: 7; (IV) a polypeptide having at least 92% sequence identity but less than 100% sequence identity to SEQ ID NO: 11 or SEQ ID NO: 12 (mature form), or a polypeptide is encoded by a polynucleotide having at least 60% sequence identity but less than 100% sequence identity to SEQ ID NO: 10; (V) a polypeptide having at least 80% sequence identity but less than 100% sequence identity to SEQ ID NO: 14 or SEQ ID NO: 15 (mature form), or a polypeptide is encoded by a polynucleotide having at least 60% sequence identity but less than 100% sequence identity to SEQ ID NO: 13; (VI) a polypeptide having at least 94% sequence identity but less than 100% sequence identity to SEQ ID NO: 17, or a polypeptide is encoded by a polynucleotide having at least 90% sequence identity but less than 100% sequence identity to SEQ ID NO: 16; (VII) a polypeptide having at least 98% sequence identity but less than 100% sequence identity to SEQ ID NO: 20 or SEQ ID NO: 21 (mature form), or a polypeptide is encoded by a polynucleotide having at least 90% sequence identity but less than 100% sequence identity to SEQ ID NO: 19; (VIII) a polypeptide having at least 82% sequence identity but less than 100% sequence identity to SEQ ID NO: 23 or SEQ ID NO: 24 (mature form), or a polypeptide is encoded by a polynucleotide having at least 70% sequence identity but less than 100% sequence identity to SEQ ID NO: 22; and (IX) a polypeptide having at least 82% sequence identity but less than 100% sequence identity to SEQ ID NO: 56 or SEQ ID NO: 57 (mature form), or a polypeptide is encoded by a polynucleotide having at least 70% sequence identity but less than 100% sequence identity to SEQ ID NO: 55; and (b) at least one cleaning component selected from a surfactant, a builder, a bleach component, a polymer, a dispersing agent and/or an additional enzyme. ### Claim 2 (depends on 1) The cleaning composition of claim 1, wherein the polypeptide has alpha-mannanase and/or alpha-mannosidase activity. ### Claim 3 (depends on 1) The cleaning composition of claim 1, wherein the polypeptide having alpha-mannan degrading activity belongs to glycosyl hydrolase family 76 (GH76). ### Claim 4 (depends on 1) The cleaning composition of claim 1, wherein the polypeptide further comprises one or more of the motifs [YND]DD[QINLEM] (SEQ ID NO: 60), GG[ILMV]X[WS] (SEQ ID NO: 61) or [RK][NLT]XXX[NTV]XP[GTLYISAVFNM] (SEQ ID NO: 64). ### Claim 5 (depends on 1) The cleaning composition claim 1, wherein the polypeptide is of the KNTPA Glade and is of bacterial origin. ### Claim 6 (depends on 1) The cleaning composition of claim 1, comprising at least two polypeptides having alpha-mannan degrading activity. ### Claim 7 (depends on 1) The cleaning composition of claim 1, wherein the polypeptide having alpha-mannan degrading activity is obtained from a strain of Bacillus or Paenibacillus. ### Claim 8 (depends on 1) The cleaning composition of claim 1, comprising at least 0.001 ppm of the polypeptide having alpha-mannan degrading activity. ### Claim 9 (depends on 1) A process for degrading alpha-mannan, comprising applying the cleaning composition of claim 1 to the alpha-mannan. ### Claim 10 (depends on 1) A method of cleaning an item, comprising the steps of: (a) contacting the item with the cleaning composition of claim 1; and (b) optionally rinsing the item, wherein the item is a textile or a hard surface. ### Claim 11 An isolated or purified polypeptide having alpha-mannan degrading activity, wherein the polypeptide is selected from the group consisting of: (I) a polypeptide having at least 96% sequence identity but less than 100% sequence identity to SEQ ID NO: 2 or SEQ ID NO: 3 (mature form), or a polypeptide is encoded by a polynucleotide having at least 90% sequence identity but less than 100% sequence identity to SEQ ID NO: 1; (II) a polypeptide having at least 70% sequence identity but less than 100% sequence identity to SEQ ID NO: 5 or SEQ ID NO: 6 (mature form), or a polypeptide is encoded by a polynucleotide having at least 60% sequence identity but less than 100% sequence identity to SEQ ID NO: 4; (III) a polypeptide having at least 75% sequence identity but less than 100% sequence identity to SEQ ID NO: 8 or SEQ ID NO: 9 (mature form), or a polypeptide is encoded by a polynucleotide having at least 60% sequence identity but less than 100% sequence identity to SEQ ID NO: 7; (IV) a polypeptide having at least 92% sequence identity but less than 100% sequence identity to SEQ ID NO: 11 or SEQ ID NO: 12 (mature form), or a polypeptide is encoded by a polynucleotide having at least 60% sequence identity but less than 100% sequence identity to SEQ ID NO: 10; (V) a polypeptide having at least 80% sequence identity but less than 100% sequence identity to SEQ ID NO: 14 or SEQ ID NO: 15 (mature form), or a polypeptide is encoded by a polynucleotide having at least 60% sequence identity but less than 100% sequence identity to SEQ ID NO: 13; (VI) a polypeptide having at least 94% sequence identity but less than 100% sequence identity to SEQ ID NO: 17, or a polypeptide is encoded by a polynucleotide having at least 90% sequence identity but less than 100% sequence identity to SEQ ID NO: 16; (VII) a polypeptide having at least 98% sequence identity but less than 100% sequence identity to SEQ ID NO: 20 or SEQ ID NO: 21 (mature form), or a polypeptide is encoded by a polynucleotide having at least 90% sequence identity but less than 100% sequence identity to SEQ ID NO: 19; (VIII) a polypeptide having at least 82% sequence identity but less than 100% sequence identity to SEQ ID NO: 23 or SEQ ID NO: 24 (mature form), or a polypeptide is encoded by a polynucleotide having at least 70% sequence identity but less than 100% sequence identity to SEQ ID NO: 22; and (IX) a polypeptide having at least 82% sequence identity but less than 100% sequence identity to SEQ ID NO: 56 or SEQ ID NO: 57 (mature form), or a polypeptide is encoded by a polynucleotide having at least 70% sequence identity but less than 100% sequence identity to SEQ ID NO: 55. ### Claim 12 (depends on 11) The polypeptide of claim 11, wherein the polypeptide having alpha-mannan degrading activity belongs to glycosyl hydrolase family 76 (GH76). ### Claim 13 (depends on 11) The polypeptide of claim 11, wherein the polypeptide further comprises one or more of the motifs [YND]DD[QINLEM] (SEQ ID NO: 60), GG[ILMV]X[WS] (SEQ ID NO: 61) or [RK][NLT]XXX[NTV]XP[GTLYISAVFNM] (SEQ ID NO: 64). ### Claim 14 (depends on 11) The polypeptide of claim 11, which is of the KNTPA Glade and is of bacterial origin. ### Claim 15 A polypeptide having at least 90% sequence identity but less than 100% sequence identity to SEQ ID NO: 9. ### Claim 16 A polypeptide having at least 90% sequence identity but less than 100% sequence identity to SEQ ID NO: 15. ### Claim 17 A polypeptide having at least 90% sequence identity but less than 100% sequence identity to SEQ ID NO: 24. ### Claim 18 A polypeptide having at least 90% sequence identity but less than 100% sequence identity to SEQ ID NO: 57.