Anti-lag-3 antibodies and methods of use thereof

An isolated antibody that specifically binds to human LAG-3, the antibody comprising a heavy chain variable region comprising complementarity determining regions CDRH1, CDRH2 and CDRH3 and a light chain variable region comprising complementarity determining regions CDRL1, CDRL2 and CDRL3, wherein:

• (a) CDRH1 comprises the amino acid sequence of DX1YX2X3(SEQ ID NO:
• 140), wherein
• X1 is T or N,
• X2 is I or M, and
• X3 is H, Y or D;
• (b) CDRH2 comprises the amino acid sequence of X1IDPANX2X3X4X5X6X7PX8X9QX10 (SEQ ID NO:
• 142), wherein
• X1 is E, R, S, or K,
• X2 is D or G,
• X3 is N or H,
• X4 is T or S,
• X5 is K or H,
• X6 is Y or F,
• X7 is D or A,
• X8 is K or R,
• X9 is F or L, and
• X10 is G or D;
• (c) CDRH3 comprises the amino acid sequence of YX1X2X3YX4VGGX5DY (SEQ ID NO:
• 144), wherein
• X1 is Y, F, or S,
• X2 is Y or D,
• X3 is K or R,
• X4 is D or E, and
• X5 is F or C;
• (d) CDRL1 comprises the amino acid sequence of SVSSX1ISSSX2LX3 (SEQ ID NO:
• 147), wherein
• X1 is S or G,
• X2 is N or T, and
• X3 is H or Y;
• (e) CDRL2 comprises the amino acid sequence of SEQ ID NO:
• 104;
• and
• (f) CDRL3 comprises the amino acid sequence of QQWX1X2YPX3T (SEQ ID NO:
• 149), wherein
• X1 is S, N, or R,
• X2 is S, T or R, and
• X3 is F, L, H, or W,
• wherein the CDRH1, CDRH2, CDRH3, CDRL1, CDRL2, and CDRL3 do not comprise the amino acid sequences of SEQ ID NOs:
• 79, 90, 98, 100, 104, and 105, respectively.