# US 11,992,844 B2 · Dried reagent strainers and methods for making and using the same

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- Number: US 11,992,844 B2
- Document ID: US11992844B2
- Status: Granted
- Kind: B2
- Application number: 16/653,390
- Filed: 2019-10-15
- Published: 2020-05-14
- Granted: 2024-05-28
- Earliest claimed: 2018-11-13
- Assignees: Becton, Dickinson and Company
- Applicants: Becton, Dickinson and Company
- Inventors: Wei Huang
- Web page: https://patentgrep.com/patent/US11992844B2

## CPC

- B01L 3/5021: {Test tubes specially adapted for centrifugation purposes}
- B01L 3/523: {with means for closing or opening}
- G01N 15/14: Optical investigation techniques, e.g. flow cytometry
- G01N 33/583: {with non-fluorescent dye label}
- B01L 2200/16: Reagents, handling or storing thereof
- B01L 2300/042: Caps; Plugs
- B01L 2300/047: Additional chamber, reservoir
- B01L 2300/0681: Filter
- B01L 2300/0829: Multi-well plates; Microtitration plates
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Dried reagent strainers, such as cell strainers are provided. Aspects of the strainers include a body having an opening with a filter positioned in the opening, where the filter includes a dried reagent composition. Aspects of the invention further include methods of making and using the strainers, as well as kits containing the strainers.

## Claims

### Claim 1

A strainer, the strainer comprising:
a body configured to mate with an open end of a liquid container and having an opening; and
a filter positioned in the opening, the filter comprising a dried reagent composition comprising a dye.

### Claim 2 (depends on 1)

The strainer according to claim 1, wherein the dye is a polymeric dye.

### Claim 3 (depends on 1)

The strainer according to claim 1, wherein the filter comprises two or more distinctly positioned dried reagent compositions.

### Claim 4 (depends on 1)

The strainer according to claim 1, wherein the dried reagent composition is positioned on only one side of the filter.

### Claim 5 (depends on 1)

The strainer according to claim 1, wherein the filter comprises a mesh.

### Claim 6 (depends on 1)

The strainer according to claim 1, wherein the strainer is configured as a cap.

### Claim 7 (depends on 6)

The strainer according to claim 6, wherein the cap is configured to snap fit or screw onto an open end of the liquid container.

### Claim 8

An assembly comprising:
a liquid container; and
a strainer, the strainer comprising:
a body mated to an open end of the liquid container and having an opening; and
a filter positioned in the opening, the filter comprising a dried reagent composition comprising a dye.

### Claim 9 (depends on 8)

The assembly according to claim 8, wherein the liquid container is configured to hold a volume ranging from 0.1 ml to 250 ml.

### Claim 10 (depends on 8)

The assembly according to claim 8, wherein the assembly further comprises a dried reagent composition inside of the liquid container.

### Claim 11 (depends on 10)

The assembly according to claim 10, wherein the dried reagent composition is positioned on an inner surface of the liquid container.

### Claim 12 (depends on 10)

The assembly according to claim 10, wherein the dried reagent composition comprises a dried reagent stably associated with a high surface area solid support.

### Claim 13 (depends on 8)

The assembly according to claim 8 further comprising a cover in operable relationship with the filter.

### Claim 14 (depends on 13)

The assembly according to claim 13, wherein the cover is opaque.

### Claim 15 (depends on 8)

A method comprising introducing a volume of a liquid into a liquid container through a strainer of an assembly according to claim 8 to produce a reconstituted reagent composition.

### Claim 16 (depends on 15)

The method according to claim 15, wherein the liquid comprises a biological sample.

### Claim 17 (depends on 16)

The method according to claim 16, wherein the biological sample comprises whole blood or a fraction thereof.

### Claim 18 (depends on 15)

The method according to claim 15, wherein the method further comprises assaying the reconstituted reagent composition.

### Claim 19 (depends on 18)

The method according to claim 18, wherein the assaying comprises flow cytometrically analyzing the reconstituted reagent composition.