# US 11,992,834 B2 · Sample analysis chip, sample analysis device containing same, and cartridge mounted on sample analysis chip

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 11,992,834 B2
- Document ID: US11992834B2
- Status: Granted
- Kind: B2
- Application number: 16/639,565
- Filed: 2018-08-24
- Published: 2021-05-06
- Granted: 2024-05-28
- Earliest claimed: 2017-08-24
- Applicants: University-Industry Cooperation Group of Kyung Hee University
- Inventors: Tae Seok Seo
- Web page: https://patentgrep.com/patent/US11992834B2

## CPC

- B01L 3/502: {with fluid transport, e.g. in multi-compartment structures}
- G01N 35/04: Details of the conveyor system {(G01N35/021 - G01N35/028 take precedence)}
- B01L 3/50273: {characterised by the means or forces applied to move the fluids}
- G01N 1/4055: {by solubility techniques}
- G01N 35/08: using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
- B01L 2200/0621: Control of the sequence of chambers filled or emptied
- B01L 2200/10: Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
- B01L 2200/16: Reagents, handling or storing thereof
- B01L 2300/0803: Disc shape
- B01L 2300/0819: Microarrays; Biochips
- B01L 2300/0864: comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
- B01L 2300/0883: Serpentine channels
- B01L 2400/0406: capillary forces
- B01L 2400/0409: centrifugal forces
- G01N 2001/4061: {Solvent extraction}
- G01N 2035/0449: {using centrifugal transport of liquid}
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A chip for sample analysis is provided. The chip for sample analysis according to an embodiment of the present application includes a sample storage unit, a capture path configured to capture an analyte contained in a sample and communicating with the sample storage unit, a washing solution storage unit communicating with the capture path and a delay unit included between the capture path and the washing solution storage unit such that a washing solution flowing from the washing solution storage unit to the capture path passes through. When the chip is rotated for sample analysis, the delay unit may be configured to delay the passage of the washing solution introduced from the washing solution storage unit such that the washing solution reaches the capture path later than the sample.

## Claims

### Claim 1

A chip for sample analysis, comprising:
a sample storage unit;
a capture path configured to capture an analyte contained in a sample and communicating with the sample storage unit;
a washing solution storage unit communicating with the capture path; and
a delay unit included between the capture path and the washing solution storage unit such that a washing solution flowing from the washing solution storage unit to the capture path passes through,
wherein, when the chip is rotated for sample analysis, the delay unit is configured to delay the passage of the washing solution introduced from the washing solution storage unit such that the washing solution reaches the capture path later than the sample,
wherein the delay unit comprises:
a delay chamber capable of storing a washing solution directed to the capture path from the washing solution storage unit;
an introduction channel included between the washing solution storage unit and the delay chamber such that the washing solution flows from the washing solution storage unit to the delay chamber; and
a delay channel included between the delay chamber and the capture path such that the washing solution stored in the delay chamber is discharged when stored at a predetermined amount or more,
wherein the delay channel comprises:
a first path extending radially inward from an outlet of the delay chamber; and
a second path extending radially outward from the end of the first path, the first path and the second path being configured to have the washing solution flow in opposite directions to each other, and
wherein a length of the first path with respect to a radially inward direction corresponds to a discharge level of the washing solution stored in the delay chamber, such that the washing solution stored in the delay chamber is discharged when having reached the discharge level or more,
wherein the introduction channel comprises:
a third path extending away from the delay chamber in a direction parallel to a width direction of the delay chamber, the third path being configured to have the washing fluid flow away from the delay chamber; and
a fourth path extending closer to the delay chamber in a direction parallel to the third path from an end of the third path, the third path and the fourth path being configured to have the washing solution flow in opposite directions to each other,
wherein the third path is connected to a farther side of the washing solution storage unit from a rotational axis of the chip, and the fourth path is positioned farther than the third path from the rotational axis of the chip and is connected to the delay chamber, which is positioned farther than the fourth path from the rotational axis of the chip,
wherein the third path only extends away from the delay chamber, and the fourth path only extends closer to the delay chamber, and
wherein the delay chamber comprises:
an outlet side formed linearly along a radial direction and connected to the first path through the outlet; and
an inlet side formed linearly and perpendicularly to the outlet side and connected to the fourth path through an inlet, the inlet side being positioned radially closer from the axis than the discharge level.

### Claim 2 (depends on 1)

The chip of claim 1, wherein the sample storage unit is located radially inward of the capture path.

### Claim 3 (depends on 1)

The chip of claim 1, wherein the washing solution storage unit is located radially inward of the sample storage unit.

### Claim 4 (depends on 3)

The chip of claim 3, further comprising:
a first eluent storage unit located radially inward of the sample storage unit, communicating with the capture path, and storing the first eluent for separating an analyte captured in the capture path therein; and
a second eluent storage unit located radially outward of the sample storage unit, and storing a second eluent for detecting the analyte therein.

### Claim 5 (depends on 4)

The chip of claim 4, further comprising:
a connection chamber located radially outward of the capture path and the second eluent storage unit, and communicating with an outlet of the capture path and the second eluent storage unit;
a waste solution chamber communicating with the connection chamber to be located radially outward of the connection chamber, and storing a sample not captured in the capture path and a washing solution passing through the capture path when the chip is rotated for sample analysis; and
a collection chamber communicating with the connection chamber to be located radially outward of the connection chamber and storing an analyte separated by the first eluent, the first eluent passing through the capture path, and the second eluent passing through the connection chamber.

### Claim 6 (depends on 4)

The chip of claim 4, wherein an injection hole for injecting a solution is formed in each of the sample storage unit, the washing solution storage unit, the first eluent storage unit and the second eluent storage unit.

### Claim 7 (depends on 1)

The chip of claim 1, wherein the outlet is included at the radially outermost side of the delay chamber, and the delay channel is connected to the outlet of the delay chamber.

### Claim 8 (depends on 1)

The chip of claim 1, further comprising:
a plurality of detection chambers communicating with the capture path, configured to introduce the analyte, and storing a different primer for detecting the analyte.

### Claim 9 (depends on 1)

A device for sample analysis, comprising:
the chip for sample analysis of claim 1;
a lower member on which the chip for sample analysis is mounted, and comprising a temperature adjustment unit on the top surface thereof;
an upper member mounted on the lower member to facilitate up-and-down movement with respect to the lower member; and
a driving motor mounted under the lower member to rotate the chip for sample analysis.