# US 11,992,523 B2 · Method for producing chikungunya virus (CHIKV) virus-like particles comprising the C, E2, and E1 structural proteins

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- Number: US 11,992,523 B2
- Document ID: US11992523B2
- Status: Granted
- Kind: B2
- Application number: 17/850,706
- Filed: 2022-06-27
- Published: 2023-01-26
- Granted: 2024-05-28
- Earliest claimed: 2008-11-26
- Assignees: The United States of America, as represented by the Secretary, Department of Health and Human Services
- Applicants: The United States of America, as represented by the Secretary, Department of Health and Human Services (the Government)
- Inventors: Gary J. Nabel; Wataru Akahata; Srinivas Rao
- Web page: https://patentgrep.com/patent/US11992523B2

## CPC

- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- A61K 39/12: Viral antigens
- A61P 29/00: Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
- A61P 31/12: Antivirals
- A61P 31/14: for RNA viruses
- C12N 7/045: {Pseudoviral particles; Non infectious pseudovirions, e.g. genetically engineered}
- A61K 2039/5258: {Virus-like particles}
- A61K 2039/55566: {Emulsions, e.g. Freund's adjuvant, MF59}
- C12N 2740/16043: viral genome or elements thereof as genetic vector
- C12N 2770/36123: Virus like particles [VLP]
- C12N 2770/36134: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The invention features compositions and methods for the prevention or treatment of one or more strains of Chikungunya virus, as well as other alphavirus-mediated diseases.

## Claims

### Claim 1

A method for producing a virus-like particle, comprising:
expressing in a cell one or more polynucleotides encoding Chikungunya virus (CHIKV) strain 37997 structural proteins under conditions for self-assembly of the CHIKV strain 37997 structural proteins to form a virus-like particle, wherein the CHIKV strain 37997 structural proteins comprise at least CHIKV capsid (C) protein, CHIKV E2 protein, and CHIKV E1 protein, and
wherein the cell does not express polynucleotides encoding CHIKV non-structural proteins.

### Claim 2 (depends on 1)

The method of claim 1, wherein the CHIKV strain 37997 structural proteins comprise at least CHIKV capsid (C) protein, CHIKV E3 protein, CHIKV E2 protein, CHIKV 6K protein, and CHIKV E 1 protein.

### Claim 3 (depends on 1)

The method of claim 1, wherein the one or more polynucleotides encode a polyprotein comprising the CHIKV capsid (C) protein, a CHIKV E3 protein, the CHIKV E2 protein, a CHIKV 6K protein, and the CHIKV E1 protein.

### Claim 4 (depends on 1)

The method of claim 1, further comprising isolating the virus-like particle.