Chimeric antigen receptor (CAR) that targets chemokine receptor CCR4 and its use

An isolated nucleic acid molecule encoding a chimeric antigen receptor, wherein the nucleic acid molecule comprises the nucleic acid sequence of SEQ ID NO:

• 11, and wherein the chimeric antigen receptor comprises:
• (a) an extracellular scFv comprising a light chain variable domain (VL) and a heavy chain variable domain (VH), wherein the scFv specifically binds to CCR4, wherein the VH comprises a heavy chain complementarity determining region (HCDR)1, an HCDR2 and an HCDR3, wherein the HCDR1 comprises amino acids 26-33 of SEQ ID NO:
• 1, the HCDR2 comprises amino acids 51-58 of SEQ ID NO:
• 1, and the HCDR3 comprises amino acids 99-107 of SEQ ID NO:
• 1, and wherein the VL comprises a light chain complementarity determining region (LCDR)1, a LCDR2 and a LCDR3, wherein the LCDR1 comprises amino acids 27-38 of SEQ ID NO:
• 2, the LCDR2 comprises amino acids 56-61 of SEQ ID NO:
• 2, and the LCDR3 comprises amino acids 95-102 of SEQ ID NO:
• 2;
• (b) a hinge and transmembrane domain from CD8;
• (c) an intracellular 4-1BB signaling domain;
• (d) an intracellular CD3 zeta signaling domain, wherein (a)-(d) are in N-terminal to C-terminal order, and
• wherein the chimeric antigen receptor further comprises an immunoglobulin signal sequence comprising the amino acid sequence of SEQ ID NO:
• 4.