Monitoring method of heart rate for toxicity test using high-speed digital holography

An individual response evaluation apparatus by real time measurement of a heart rate of an aquatic organism, using digital holography, the apparatus comprising:

• a chamber carrying a culture fluid;
• a lower fixing unit;
• an upper fixing unit spaced apart from the lower fixing unit;
• and a control unit controlling a position of the upper fixing unit,
• wherein the lower fixing unit is fixed to a lower end inside the chamber, an aquatic organism is disposed between the lower fixing unit and the upper fixing unit, the upper fixing unit is fixed to one side surface of the chamber and movable up and down, and the control unit adjusts the position of the upper fixing unit to fix the aquatic organism, after the upper fixing unit is adhered to the aquatic organism to measure the height the aquatic organism, the apparatus further comprising:
• a light source unit;
• a reference beam generating unit which produces a reference beam;
• an object beam generating unit which produces an object beam;
• a CCD which combines the reference beam and the object beam to record a hologram;
• an arithmetic unit which numerically analyzes an interference fringe of the recorded hologram;
• a display unit which displays the digital hologram numerically analyzed by the arithmetic unit;
• and a heart rate analysis unit which carries out heart rate analysis from the analyzed digital hologram,
• wherein the arithmetic unit obtains a plurality of phase restoration phases for the hologram recorded in the CCD, and calculates an amplitude and an angle therefrom and transfers calculations to the display unit, the display unit selects and displays the corresponding digital hologram until analysis for all holograms recorded in the CCD is completed and then transfers the digital hologram to the heart rate analysis unit, and in the heart rate analysis, a heart rate per minute is calculated from the most frequent heart rate, and
• wherein the heart rate is obtained by an analysis method including:
• a) selecting pixels corresponding to a heart area in a secured digital hologram;
• b) applying a Fourier spectrum method to each refreshing interval;
• c) searching a most frequent heart rate in each refreshing interval;
• and
• d) calculating a heart rate per minute from the most frequent heart rate.