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US 11,988,666 B2Granted
Lectin-binding substance measurement method, lectin-binding substance measurement kit, and blocked labeled lectin for use in these
AssigneeFujirebio Inc.
InventorsTomonori Nishii, Kazunori Okada, Naoki Ishikawa, Kumiko Iida, Shintaro Yagi, Katsumi Aoyagi
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Abstract
A lectin-binding substance measurement method is a method for measuring a lectin-binding substance in a sample, and includes a measuring step of bringing a blocked labeled lectin including a water-soluble carrier made of a first water-soluble polymer and a labeling substance and a lectin immobilized on the water-soluble carrier into contact with the sample.
Claims17
A lectin-binding substance measurement method for measuring a lectin-binding substance in a sample, the method comprising
- a measuring step of bringing a blocked labeled lectin and a labeled lectin into contact with the sample, wherein
- the blocked labeled lectin is a combination of
- a high molecular weight blocked labeled lectin including a first water-soluble carrier made of a high molecular weight water-soluble polymer having a weight average molecular weight of 200,000 or more, and a first labeling substance and a first lectin immobilized on the first water-soluble carrier and
- a low molecular weight blocked labeled lectin including a second water-soluble carrier made of a low molecular weight water-soluble polymer having a weight average molecular weight of less than 100,000, and a second labeling substance and a second lectin immobilized on the second water-soluble carrier,
- the labeled lectin comprises a third labeling substance and a third lectin, and excludes any water-soluble carrier,
- the high molecular weight water-soluble polymer and the low molecular weight water soluble polymer are comprised of a same or different class of polymer,
- the first labeling substance, the second labeling substance, and the third labeling substance are a same or different labeling substance, and
- the first lectin, the second lectin, and the third lectin are a same or different lectin.
5 more elements
Dependencies are inferred from the claim text.