Methods for tracking improved athletic performance by detecting expression of dysferlin
AssigneeThe Translational Genomics Research Institute
InventorMatthew Huentelman
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method of creating a biomarker profile, the method comprising the steps of: obtaining a sample of biofluid from a subject, wherein the sample is stored on a sample collection apparatus; removing the sample from the sample collection apparatus; extracting nucleic acids from the sample; sequencing the extracted nucleic acids to generate sequence data; and analyzing the sequence data using a two-step analytical methodology to create the biomarker profile. The present invention is also directed to methods of determining the sex of an in utero fetus, predicting onset of a migraine in a subject, and of tracking athletic performance in a subject.
Claims12
A method of tracking improved athletic performance in a subject, the method comprising the steps of:
- obtaining a set of samples of biofluid from the subject collected before, during and/or after aerobic exercise and stored on sample collection apparatuses, wherein the biofluid is selected from the group consisting of blood, plasma, and serum;
- the sample collection apparatus comprises a cellulose paper on which the biofluid is placed to dry, and the cellulose paper has not been treated with any chemical stabilizers of nucleic acids;
- extracting nucleic acids from the set of samples, wherein the nucleic acids are RNA;
- sequencing the extracted nucleic acids to generate sequence data, wherein the RNA is sequenced with RNA-seq;
- and
- analyzing the sequence data to identify increases in gene expression of dysferlin (DYSF), wherein an increase in expression of DYSF in the sample collected during or after aerobic exercise compared to the sample collected before aerobic exercise indicates improved athletic performance;
- wherein the sample collected during or after aerobic exercise was obtained from about 1 hour after beginning aerobic exercise to about 4 hours from completion of the aerobic exercise.
A method of tracking athletic performance in a subject, the method comprising the steps of:
- obtaining a set of samples of biofluid from the subject collected before, during and/or after aerobic exercise and stored on sample collection apparatuses, wherein the biofluid is selected from the group consisting of blood, plasma, and serum;
- the sample collection apparatus comprises a cellulose paper on which the biofluid is placed to dry, and the cellulose paper has not been treated with any chemical stabilizers of nucleic acids;
- extracting nucleic acids from the set of samples, wherein the nucleic acids are RNA;
- sequencing the extracted nucleic acids to generate sequence data, wherein the RNA is sequenced with RNA-seq;
- and
- analyzing the sequence data to identify increases in gene expression of dysferlin (DYSF) and matrix metallopeptidase 9 (MMP9) in the sample collected during or after exercise, wherein an increase in expression of DYSF and MMP9 compared to the sample collected before aerobic exercise indicates improved athletic performance;
- wherein the sample collected during or after aerobic exercise was obtained from about 1 hour after beginning aerobic exercise to about 4 hours from completion of the aerobic exercise.
Dependencies are inferred from the claim text.