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US 11,987,843 B2Granted
Bisulfite-free, base-resolution identification of cytosine modifications
AssigneeLudwig Institute for Cancer Research
InventorsChunxiao Song, Yibin Liu
Life of this patent
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Abstract
This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.
Claims18
A method for converting a modified cytosine to dihydrouracil (DHU) comprising:
- i) contacting a nucleic acid sample comprising a modified cytosine with an oxidizing agent to produce 5-carboxylcytosine (5caC) and/or 5-formylcytosine (5fC);
- and
- ii) contacting the nucleic acid sample comprising 5caC and/or 5fC with a borane reducing agent.
Dependencies are inferred from the claim text.