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US 11,987,834 B2Granted
Acetaminophen adducts and methods of use thereof
AssigneeBioVentures, LLC
InventorsLaura P. James, Jack Hinson, Dean Roberts, Pritmohinder S. Gill
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Abstract
The present disclosure relates to acetaminophen protein adducts and methods of diagnosing acetaminophen toxicity using the acetaminophen protein adducts.
Claims7
A method for measuring the amount of an acetaminophen-protein adduct comprising a protein bound to acetaminophen in a biological sample, the method comprising:
- a) providing a biological sample from a subject having or suspected of having acetaminophen toxicity;
- b) contacting the sample with a first antibody specific to the protein to form a first complex between the first antibody and the protein, wherein the protein is selected from the group consisting of betaine-homocysteine S-methyltransferase 1, cytoplasmic aspartate aminotransferase, 1,4-alpha-glucan-branching enzyme, formimidoyltransferase-cyclodeaminase, and dystrophin;
- c) isolating the first complex;
- d) contacting the isolated first complex with a second antibody specific to a 3-(cystein-S-yl) APAP (3-Cys-A)-protein linkage on the acetaminophen-protein adduct to form a second complex;
- e) measuring the amount of the second complex, wherein the amount of the second complex is indicative of the amount of the acetaminophen-protein adduct.
2 more elements
Dependencies are inferred from the claim text.