Method of manufacturing cell spheroid using bioink
AssigneeUnist (Ulsan National Institute of Science and Technology)
InventorsHyun Wook Kang, Seug Gyu Jeon, Jun Ho Heo
Life of this patent
Estimating the term…
Abstract
Disclosed is a process of manufacturing cell spheroids using a bioink. More particularly, provided is a method of manufacturing a cell spheroid, the method including extruding a first bioink including an alginate; extruding a second bioink including cells into the extruded first bioink; adding a calcium chloride (CaCl2) solution to the alginate included in the first bioink; and dissolving the second bioink, present in the first bioink, in a cell culture medium to form a cell spheroid from the cells.
Claims15
A method of manufacturing a cell spheroid, the method comprising:
- extruding a first bioink that is composed of a substrate excluding cells and comprising an alginate;
- forming a spherical space inside the first bioink by extruding a second bioink comprising dispersed cells into the first bioink;
- adding a calcium chloride (CaCl2) solution to the alginate comprised in the first bioink, wherein calcium chloride in the CaCl2 solution is crosslinked with the alginate of the first bioink;
- and
- dissolving the second bioink, present in the first bioink, in a cell culture medium to form a cell spheroid from the cells of the second bioink.
Dependencies are inferred from the claim text.