Attb cell line, transgenic cell lines derived therefrom, and methods of making the same
AssigneeAlbert Einstein College of Medicine
InventorsWilliam R. Jacobs, Jr., Lawrence Leung, Regy Lukose, Anna Paula de Oliveira
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Abstract
A genetically modified mammalian cell and genetically modified mammalian cell line comprise a recombination sequence inserted in a target locus on a chromosome of the mammalian cell genome, wherein the recombination sequence comprises Bxb1attB sequence from Mycobacterium smegmatis. A transgenic mammalian cell and transgenic mammalian cell line comprise a heterologous nucleic acid stably integrated in a target locus on a chromosome of the mammalian genome, wherein the heterologous nucleic comprises a heterologous gene configured for expression by the transgenic mammalian cell.
Claims44
A genetically modified mammalian cell comprising a recombination sequence inserted in a target locus on a chromosome of the Vero cell genome, wherein the recombination sequence comprises a Bxb1 attB sequence from Mycobacterium smegmatis wherein the mammalian cell is a Vero cell and the target locus comprises adeno-associated virus integration site 1 (AAVS1) on chromosome 6 of the Vero cell genome, wherein the AAVS1 locus comprising the recombination sequence has the 4 kilobase sequence of SEQ ID NO:22.
Dependencies are inferred from the claim text.