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US 11,987,709 B2Granted
Silicon containing detectable compounds and uses thereof
AssigneeSingular Genomics Systems, Inc.
InventorsMegha Cila, Ronald Graham, Rodrigo Rodriguez
Life of this patent
Estimating the term…
Abstract
Disclosed herein, inter alia, are silicon containing detectable compounds and methods of use thereof. In an aspect is provided a monovalent nucleotide or monovalent nucleoside covalently bound to a monovalent form of a compound described herein (e.g., wherein the R13 moiety of a compound described herein has reacted with a bioconjugate reactive group to form a bioconjugate linker thereby covalently bonding the monovalent compound to the monovalent nucleotide or monovalent nucleoside).
Claims21
An agent covalently bound to a compound, or salt thereof, via a bioconjugate linker, wherein said agent is a protein, carbohydrate, or virus, and said compound or salt thereof has the formula:
- wherein
- R1, R8, R9, R10, R11, and R12 are independently hydrogen, halogen, —CCl3, —CBr3, —CF3, —CI3, —CHCl2, —CHBr2, —CHF2, —CHI2, —CH2Cl, —CH2Br, —CH2F, —CH2I, —CN, —OH, —NH2, —COOH, —CONH2, —NO2, —SH, —SO3H, —SO4H, —SO2NH2, —NHNH2, —ONH2, —NHC(O)NHNH2, —NHC(O)NH2, —NHSO2H, NHC(O)H, —NHC(O)OH, —NHOH, —OCCl3, —OCF3, —OCBr3, —OCI3, —OCHCl2, —OCHBr2, —OCHI2, —OCHF2, —OCH2Cl, —OCH2Br, —OCH2I, —OCH2F, —N3, —SF5, —PO3H, substituted or unsubstituted alkyl, or substituted or unsubstituted heteroalkyl, or a bioconjugate linker;
- R2 and R3 are independently substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, or a bioconjugate linker;
- R4, R5, R6, and R7 are independently hydrogen, halogen, —CF3, —CBr3, —CCl3, —CI3, —CHF2, —CHBr2, —CHCl2, —CHI2, —CH2F, —CH2Br, —CH2Cl, —CH2I, —OH, —COOH, —CONH2, —SH, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, or a bioconjugate linker;
- R4 and R5 substituents bonded to the same nitrogen atom may optionally be joined to form a substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R6 and R7 substituents bonded to the same nitrogen atom may optionally be joined to form a substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R4 and R9 substituents may optionally be joined to form a substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R7 and R16 substituents may optionally be joined to form a substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R5 and R11 substituents may optionally be joined to form a substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R6 and R12 substituents may optionally be joined to form a substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R1 and R9 substituents may optionally be joined to form a substituted or unsubstituted cycloalkyl, substituted or unsubstituted aryl;
- substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R8 and R10 substituents may optionally be joined to form a substituted or unsubstituted cycloalkyl, substituted or unsubstituted aryl;
- substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- wherein one of R1, R2, R3, R4, R5, R6, R7, R8, R9, R10, R11, and R12 is a bioconjugate linker.
12 more elements
A compound, or salt thereof, having the formula:
- wherein
- R1, R8, R9, R10, R11, and R12 are independently L1-R13, hydrogen, halogen, —CCl3, —CBr3, —CF3, —CI3, —CHCl2, —CHBr2, —CHF2, —CHI2, —CH2Cl, —CH2Br, —CH2F, —CH2I, —CN, —OH, —NH2, —COOH, —CONH2, —NO2, —SH, —SO3H, —SO4H, —SO2NH2, —NHNH2, —ONH2, —NHC(O)NHNH2, —NHC(O)NH2, —NHSO2H, NHC(O)H, —NHC(O)OH, —NHOH, —OCCl3, —OCF3, —OCBr3, —OCI3, —OCHCl2, —OCHBr2, —OCHI2, —OCHF2, —OCH2Cl, —OCH2Br, —OCH2I, —OCH2F, —N3, —SF5, —PO3H, substituted or unsubstituted alkyl, or substituted or unsubstituted heteroalkyl;
- R2 and R3 are independently L1-R13, substituted or unsubstituted alkyl, or substituted or unsubstituted heteroalkyl;
- R4, R5, R6, and R7 are independently -L1-R13, hydrogen, halogen, —CF3, —CBr3, —CCl3, —CI3, —CHF2, —CHBr2, —CHCl2, —CHI2, —CH2F, —CH2Br, —CH2Cl, —CH2I, —OH, —COOH, —CONH2, —SH, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl;
- R4 and R9 substituents may optionally be joined to form a substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R7 and R10 substituents may optionally be joined to form a substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R5 and R11 substituents may optionally be joined to form a substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R6 and R12 substituents may optionally be joined to form a substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R1 and R9 substituents may optionally be joined to form a substituted or unsubstituted cycloalkyl, substituted or unsubstituted aryl;
- substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R8 and R10 substituents may optionally be joined to form a substituted or unsubstituted cycloalkyl, substituted or unsubstituted aryl;
- substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- wherein one of R1, R2, R3, R4, R5, R6, R7, R8, R9, R10, R11, and R12 is L1-R13;
- R13 is —NH2,
- and
- L1 is a covalent linker or a bond.
13 more elements
A nucleotide bound to a monovalent compound, wherein said monovalent compound has the formula:
- wherein,
- R1, R8, R9, R10, R11, and R12 are independently hydrogen, halogen, —CCl3, —CBr3, —CF3, —CI3, —CHCl2, —CHBr2, —CHF2, —CHI2, —CH2Cl, —CH2Br, —CH2F, —CH2I, —CN, —OH, —NH2, —COOH, —CONH2, —NO2, —SH, —SO3H, —SO4H, —SO2NH2, —NHNH2, —ONH2, —NHC(O)NHNH2, —NHC(O)NH2, —NHSO2H, NHC(O)H, —NHC(O)OH, —NHOH, —OCCl3, —OCF3, —OCBr3, —OCI3, —OCHCl2, —OCHBr2, —OCHI2, —OCHF2, —OCH2Cl, —OCH2Br, —OCH2I, —OCH2F, —N3, —SF5, —PO3H, substituted or unsubstituted alkyl, or substituted or unsubstituted heteroalkyl;
- R2 and R3 are independently substituted or unsubstituted alkyl, or substituted or unsubstituted heteroalkyl;
- R4, R5, R6, and R7 are independently hydrogen, halogen, —CF3, —CBr3, —CCl3, —CI3, —CHF2, —CHBr2, —CHCl2, —CHI2, —CH2F, —CH2Br, —CH2Cl, —CH2I, —OH, —COOH, —CONH2, —SH, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl;
- R4 and R5 substituents bonded to the same nitrogen atom may optionally be joined to form a substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R6 and R7 substituents bonded to the same nitrogen atom may optionally be joined to form a substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R4 and R9 substituents may optionally be joined to form a substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R7 and R10 substituents may optionally be joined to form a substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R5 and R11 substituents may optionally be joined to form a substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R6 and R12 substituents may optionally be joined to form a substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R1 and R9 substituents may optionally be joined to form a substituted or unsubstituted cycloalkyl, substituted or unsubstituted aryl;
- substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- R8 and R10 substituents may optionally be joined to form a substituted or unsubstituted cycloalkyl, substituted or unsubstituted aryl;
- substituted or unsubstituted heterocycloalkyl, or substituted or unsubstituted heteroaryl;
- and
- L1 is a covalent linker.
13 more elements
Dependencies are inferred from the claim text.