Solid support for synthesizing nucleic acid sequences and methods for making and using

A solid support according to Formula I

• wherein:
• CPG is controlled pore glass;
• m is 2, 3, 4, 5 or 6;
• x is 1, 2, 3, 4 or 5;
• y is from 2 to 12;
• n is 3, 4, 5, 6, 7, 8, 9 or 10;
• R2 is H,
• p is 2, 3, 4, 5, 6, 7, 8, 9 or 10;
• R3 is H or
• R4 is H or OR6;
• R is PG or a nucleic acid sequence;
• R6 is pixyl, TBDMS, TBDPS, TMS, TES, or TIPS;
• T is 1, 2, 3, or 4;
• BP is a nucleic acid base where an exocyclic amine, if present, is protected;
• PG is a protecting group;
• and
• each R1 independently is a thermolabile phosphate protecting group having a structure
• wherein:
• X is O or S;
• R7 is H, Ra, ORa, SRa, or N(Rb)2;
• Ra is Rd;
• Rb is H, Rd or two Rbs together with the nitrogen to which they are attached, form a 3- to 7-membered heterocyclyl;
• Z is O, S, N(Rc), C(Rc)2 or C(Rc)2C(Rc)2;
• each Rc independently is H or Rd, or one Rd in combination with the C═X moiety and one Ra or Rb from R7 together form a 3- to 7-membered cycloaliphatic or heterocyclyl ring;
• Rd is alkyl, alkenyl, alkynyl, cycloalkyl, aryl, or aralkyl;
• each R8 independently is H or Rd, or one R8 together with Z forms an aryl ring;
• each R9 independently is H or Rd, or one R9 and one R8 together with the atoms to which they are attached, forms a moiety having a formula
• wherein r is 0 to 6;
• and
• each R10 independently is H, C1-6alkyl, NO2, —N(C1-6alkyl)2, —OC1-6alkyl, —SC1-6alkyl, —CN, or halogen, provided that the aromatic ring substituted with R10 is one carbon removed from the phosphate oxygen of Formula I.