Identification of a T cell epitope of Prevotella copri that induces T cell responses in patients with rheumatoid arthritis
AssigneeThe General Hospital Corporation
InventorsAllen C. Steere, Elise E. Drouin
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Abstract
Disclosed herein are methods of diagnosing Rheumatoid arthritis in a subject comprising determining whether the subject is immunologically reactive with P. copri or a portion thereof. In one aspect of the invention the immunological reactivity of the subject to one or more protein of P. copri or polypeptide fragments thereof such as protein Pc-p27 or polypeptide fragments thereof, as compared to an appropriate control, indicates the subject has rheumatoid arthritis. Examples of specific assays, compositions, and kits for use with the methods are also disclosed.
Claims14
A method for determining whether a biological sample obtained from a subject is immunologically reactive with Prevotella copri (P. copri) Pc-p27 protein or antigen thereof, the method comprising:
- (a) performing an assay for identifying the presence of antibodies that specifically bind the P. copri Pc-p27 protein or antigen thereof using a kit comprising one or more polypeptides comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, and combinations thereof, and reagents for conducting an assay for detecting the presence of an antibody in a sample that binds to the one or more polypeptides, wherein the one or more polypeptides are attached to a support matrix or further comprise a detectable marker;
- or
- (b) performing an assay for identifying T cells specifically reactive to the P. copri Pc-p27 protein or antigen thereof using a composition comprising one or more polypeptides comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, and combinations thereof;
- and
- (c) determining the biological sample is reactive with the P. copri Pc-p27 protein or antigen thereof when either assay produces positive results compared to an appropriate control sample.
A kit comprising, one or more antigens of P. copri, wherein the antigen comprises an amino acid sequence of a polypeptide of P. copri, wherein the polypeptide of P. copri is selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, and combinations thereof, and reagents for conducting an assay for detecting the presence of an antibody in a sample that binds to the one or more antigens, wherein the antigen is attached to a support matrix or further comprises a detectable marker.
A composition comprising an antigen of P. copri attached to a support matrix, wherein the antigen comprises an amino acid sequence of a polypeptide of P. copri, wherein the polypeptide of P. copri is selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, and combinations thereof.
Dependencies are inferred from the claim text.