3D cell washer

A microplate cell washing system comprising:

• a microplate carrier configured to support a microplate, the microplate comprising a plurality of microplate wells;
• a dispensing pump;
• a dispensing pump cassette comprising a plurality of dispense lines connected to the dispensing pump and terminating in dispense pipes connected to the plurality of dispense lines, wherein the dispensing pump is configured to control a first flow rate of a solution from a fluid reservoir through the plurality of dispense lines;
• an aspirating peristaltic pump;
• an aspirating peristaltic pump cassette comprising a plurality of aspiration lines directly connected to the aspirating peristaltic pump and terminating in aspiration pipes connected to the plurality of aspiration lines, wherein the aspirating peristaltic pump is configured to control a second flow rate of a waste solution through the plurality of aspiration lines;
• a processor configured to execute a cleaning control program, which when executed causes the microplate cell washing system to independently control:
• the dispensing pump to control the first flow rate of the solution from the fluid reservoir through the plurality of dispense lines for output via the dispense pipes in microplate wells corresponding to positions of the dispense pipes, and
• the aspirating peristaltic pump to control the second flow rate of the waste solution through the plurality of aspiration lines for aspiration via the aspiration pipes in microplate wells corresponding to positions of the aspiration pipes.