BAHD acyltransferases
AssigneesWisconsin Alumni Research Foundation, The University of British Columbia
InventorsSteven D. Karlen, Rebecca Anne Smith, John Ralph, Emily Beebe, Craig Bingman, Brian Fox, Shawn Mansfield, Heather Mackay, Hoon Kim, Yaseen Mottiar, Faride Unda
Life of this patent
Estimating the term…
Abstract
The invention is directed to BAHD acyltransferase enzymes, nucleic acids encoding BAHD acyltransferase enzymes, and inhibitory nucleic acids adapted to inhibit the expression and/or translation of BAHD acyltransferase RNA; expression cassettes, plant cells, and plants that have or encode such nucleic acids and enzymes; and methods of making and using such nucleic acids, enzymes, expression cassettes, cells, and plants.
Claims63
A recombinant nucleic acid comprising a nucleic acid segment that encodes:
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:2 that exhibits p-hydroxybenzoyl-CoA:monolignol transferase (pBMT) activity, feruloyl-CoA:monolignol transferase (FMT) activity, p-coumaroyl-CoA:monolignol transferase (PMT) activity, acetyl-CoA:monolignol transferase (AMT) activity, benzoyl-CoA:monolignol transferase (BMT) activity, or any combination thereof;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:4 that exhibits pBMT activity, AMT activity, BMT activity, or any combination thereof;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:6 that exhibits pBMT activity, AMT activity, BMT activity, or any combination thereof;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:8 that exhibits FMT activity, PMT activity, BMT activity, or any combination thereof;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:12 that exhibits pBMT activity;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:14 that exhibits FMT activity, PMT activity, or any combination thereof;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:16 that exhibits FMT activity, PMT activity, or any combination thereof;
- or
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:18 that exhibits FMT activity,
- wherein the nucleic acid segment is operably linked to a heterologous promoter, wherein the promoter is a promoter functional or active during plant development or growth.
A recombinant plant cell comprising a recombinant nucleic acid, wherein:
- the recombinant nucleic acid comprises a nucleic acid segment that encodes;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:2 that exhibits p-hydroxybenzoyl-CoA:monolignol transferase (pBMT) activity, feruloyl-CoA:monolignol transferase (FMT) activity, p-coumaroyl-CoA:monolignol transferase (PMT) activity, acetyl-CoA:monolignol transferase (AMT) activity, benzoyl-CoA:monolignol transferase (BMT) activity, or any combination thereof;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:4 that exhibits pBMT activity, AMT activity, BMT activity, or any combination thereof;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:6 that exhibits pBMT activity, AMT activity, BMT activity, or any combination thereof;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:8 that exhibits FMT activity, PMT activity, BMT activity, or any combination thereof;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:12 that exhibits pBMT activity;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:14 that exhibits FMT activity, PMT activity, or any combination thereof;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:16 that exhibits FMT activity, PMT activity, or any combination thereof;
- or
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:18 that exhibits FMT activity;
- the nucleic acid segment is operably linked to a heterologous promoter functional or active during plant development or growth;
- and
- the plant cell comprises a genome stably transformed with the recombinant nucleic acid.
A plant comprising a recombinant nucleic acid, wherein the recombinant nucleic acid comprises a nucleic acid segment that encodes a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, or SEQ ID NO:18, wherein the nucleic acid segment is operably linked to a heterologous promoter functional or active during plant development or growth, and wherein the plant comprises lignin having a modified content of monolignol ester conjugates compared to a control plant of the same species lacking the recombinant nucleic acid.
A plant seed comprising a recombinant nucleic acid, wherein:
- the recombinant nucleic acid comprises a nucleic acid segment that encodes:
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:2 that exhibits p-hydroxybenzoyl-CoA:monolignol transferase (pBMT) activity, feruloyl-CoA:monolignol transferase (FMT) activity, p-coumaroyl-CoA:monolignol transferase (PMT) activity, acetyl-CoA:monolignol transferase (AMT) activity, benzoyl-CoA:monolignol transferase (BMT) activity, or any combination thereof;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:4 that exhibits pBMT activity, AMT activity, BMT activity, or any combination thereof;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:6 that exhibits pBMT activity, AMT activity, BMT activity, or any combination thereof;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:8 that exhibits FMT activity, PMT activity, BMT activity, or any combination thereof;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:12 that exhibits pBMT activity;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:14 that exhibits FMT activity, PMT activity, or any combination thereof;
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:16 that exhibits FMT activity, PMT activity, or any combination thereof;
- or
- a BAHD acyltransferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:18 that exhibits FMT activity;
- and
- the nucleic acid segment is operably linked to a heterologous promoter functional or active during plant development or growth.
Dependencies are inferred from the claim text.