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US 11,980,207 B2Granted
Recombinant components and compositions for use in food products
AssigneePerfect Day, Inc.
InventorsTimothy Geistlinger, Heather Jensen, Ravirajsinh Jhala, Hendrik Meerman, Balakrishnan Ramesh, Ty Wagoner, Timothy Scott Johnson, Vincent Wei-Xiang Wu, Francesca Manea
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Abstract
Provided are methods for producing food products comprising recombinant components, and compositions used in and food products produced by such methods.
Claims31
A method for producing a food product comprising a recombinant component, the method comprising:
- providing a recombinant microbial host cell genetically modified to:
- i) decrease or eliminate expression of a gene encoding a cutinase as compared to expression of the gene in an identical unmodified microbial host cell wherein the decreased or eliminated expression is due to a genetic modification in the gene encoding the cutinase, and wherein the cutinase is capable of esterase-catalyzed release of free fatty acids in a food product, and
- ii) produce the recombinant component, wherein the genetic modification to produce the recombinant component increases production of the recombinant component as compared to production of the recombinant component in an identical unmodified microbial host cell;
- allowing the recombinant microbial host cell to produce the recombinant component, and optionally purifying the recombinant component, wherein the recombinant component is suitable for incorporation into a food product;
- and
- incorporating the recombinant component into a food product comprising at least one of a diglyceride, a triglyceride, a phospholipid, and a lipoprotein.
3 more elements
Dependencies are inferred from the claim text.