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US 11,976,383 B2Granted
Integral membrane protein display on poxvirus extracellular enveloped virions
AssigneeVaccinex, Inc.
InventorsErnest S. Smith, Maria G. M. Scrivens, Loretta Mueller, Shuying Shi, Leslie A. Balch
Life of this patent
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Abstract
This disclosure provides compositions and methods for expressing and displaying isolated integral membrane proteins (IMPs) or fragments thereof in a native conformation on poxvirus extracellular virions and methods for screening, selecting, and identifying antibodies or antibody-like molecules that bind to a target IMP of interest.
Claims31
An isolated polynucleotide comprising:
- (a) a first nucleic acid fragment that encodes an integral membrane protein (IMP) or fragment thereof, wherein the IMP or fragment thereof comprises at least one extra-membrane region, at least one transmembrane domain and at least one intra-membrane region, and wherein a portion of the first nucleic acid fragment encoding at least one intra-membrane region is situated at the 5′ or 3′ end of the first nucleic acid fragment;
- and (b) a second nucleic acid fragment that encodes a fowlpox virus (FPV) FPV108 protein or functional fragment thereof, wherein the second nucleic acid fragment is fused in frame to a portion of the first nucleic acid fragment that encodes an intra-membrane region of the IMP;
- wherein a poxvirus infected cell comprising the polynucleotide can express an IMP-FPV108 fusion protein as part of the outer envelope membrane of an extracellular enveloped virion (EEV).
Dependencies are inferred from the claim text.