Non-integrating viral delivery system and methods related thereto
AssigneeAmerican Gene Technologies International Inc.
InventorsCharles David Pauza, Tyler Lahusen
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Abstract
A non-integrating viral delivery system is disclosed. The system includes a viral carrier, wherein the viral carrier contains a defective integrase gene; a heterologous viral episomal origin of replication; a sequence encoding at least one initiator protein specific for the heterologous viral episomal origin of replication, wherein expression of the sequence encoding the at least one initiator protein specific for the heterologous viral episomal origin of DNA replication is inducible; and at least one gene, gene product, shRNA, siRNA, miRNA, or other RNA of interest.
Claims12
A non-integrating viral delivery system, the system comprising:
- a viral carrier, wherein the viral carrier contains a defective integ rase gene;
- a heterologous viral episomal origin of DNA replication;
- a first sequence encoding at least one initiator protein specific for the heterologous viral episomal origin of DNA replication, wherein expression of the first sequence is inducible;
- and
- a second sequence encoding at least one gene, gene product, shRNA, siRNA, miRNA, or other RNA of interest,
- wherein the at least one initiator protein, when expressed, functions to regulate expression of the second sequence;
- and
- wherein the heterologous viral episomal origin of DNA replication comprises a sequence having at least 95% sequence identity with SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.