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US 11,976,286 B2Granted
Modulation of nitrate levels in plants via mutation of nitrate reductase
AssigneePhilip Morris Products S.A.
InventorsLucien Bovet, Prisca Campanoni, Simon Goepfert
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Abstract
A plant cell comprising: (a) a polynucleotide sequence encoding a nitrate reductase polypeptide comprising a contiguous polypeptide sequence of SEQ ID NO: 5 or SEQ ID NO: 7, wherein methionine is substituted for an amino acid that reduces nitrate reductase activity in the plant cell as compared to a control plant cell; (b) a polypeptide sequence encoded by the polynucleotide sequence set forth in (a); or (c) a construct, vector or expression vector comprising the polynucleotide sequence set forth in (b).
Claims19
A Nicotiana tabacum plant cell comprising:
- a genome comprising a polynucleotide sequence encoding a nitrate reductase polypeptide comprising the polypeptide sequence of SEQ ID NO:
- 5 except that the methionine in SEQ ID NO:
- 5 is substituted for an amino acid that reduces nitrate levels in the plant cell as compared to a control Nicotiana tabacum plant cell, wherein the amino acid is glycine, ala nine, proline, isoleucine, leucine, or valine,
- wherein the Nicotiana tabacum plant cell is homozygous for the substitution to the amino acid.
1 more element
Dependencies are inferred from the claim text.