US 11,976,092 B2Granted

RNA nanostructures, methods of making, and uses thereof

AssigneesOhio State Innovation Foundation, University of Kentucky Research Foundation

InventorsPeixuan Guo, Mario Vieweger, Xin Li, Sijin Guo, Hongran Yin, Xijun Piao, Yi Shu, Dan Shu, Mehdi Rajabi

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Abstract

Disclosed herein are high Tm RNA nanostructures that can be composed of one or more modules or motifs to build RNA nanostructures with or without layers. The RNA nanostructures can have a core domain and three or more double-stranded arms and formulations thereof to conjugate high copy numbers of therapeutics, pH responsive or enzyme cleavable drug cargo. Also described herein is a design strategy for generation of synthetic RNA oligonucleotides that can self assemble into highly thermostable RNA structures. Also described herein are uses of the RNA nanostructures described herein.

Claims19

Dependent claims

An RNA nanostructure comprising:

at least three synthetic RNA oligonucleotides,
wherein the at least three synthetic RNA oligonucleotides are coupled to each other to form a central core domain and at least three double-stranded arms arranged around the core domain and extending away from the central core domain,
wherein at least 3 cargo compound molecules are conjugated to each of the at least three synthetic RNA oligonucleotides,
wherein the melting temperature of the RNA nanostructure with the conjugated 3 to 100 cargo compounds is greater than 65 degrees Celcius, and
wherein the at least three synthetic RNA oligonucleotides are configured to self-assemble to form the RNA nanostructure.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application: 16/635,312Filed 12 Nov 2018 · us provisional application
Priority: 9 Feb 2018
Published: US 2020/0369710 A126 Nov 2020
Granted: 7 May 2024
Term ends: Not estimatedThe source records don’t support an estimate.

Classification22 codes

C07H 21/02
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of…: with…
C12N 15/111
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: general methods applicable to biologically…
A61K 47/549
The modifying agent being an organic compound: sugars, nucleosides, nucleotides or nucleic acids
A61K 47/55
The modifying agent being an organic compound: the modifying agent being also a pharmacologically or therapeutically active agent, i.e. the entire…