Recombinant chimeric bovine/human parainfluenza virus 3 expressing rsv g and its use
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsPeter L. Collins, Ursula J. Buchholz, Bo Liang, Shirin Munir
Life of this patent
Estimating the term…
Abstract
Recombinant chimeric bovine/human parainfluenza virus 3 (rB/HPIV3) vectors expressing Respiratory Syncytial Virus (RSV) G protein or a recombinant RSV G protein, as well as methods of their use and manufacture, are provided. The rB/HPIV3 vector comprises a genome comprising a heterologous gene encoding the RSV G protein or the recombinant RSV G protein. Nucleic acid molecules comprising the sequence of the genome or antigenome of the disclosed rB/HPIV3 vectors are also provided. The disclosed rB/HPIV3 vectors can be used, for example, to induce an immune response to RSV and HPIV3 in a subject.
Claims20
A recombinant chimeric bovine/human parainfluenza virus 3 (rB/HPIV3), comprising:
- a genome comprising, in a 3′ to 5′ order, a 3′ leader region, a BPIV3 N gene, a heterologous gene, BPIV3 P and M genes, HPIV3 F and HN genes, a BPIV3 L gene, and a 5′ trailer region;
- wherein the heterologous gene encodes one of:
- (a) a RSV G protein comprising an RSV G ectodomain, transmembrane domain, and cytoplasmic tail, wherein the heterologous gene is codon optimized for human expression and comprises an antigenomic cDNA sequence set forth as SEQ ID NO:
- 89 or 90;
- (b) a recombinant RSV G protein comprising a RSV G ectodomain, a BPIV3 HN transmembrane domain, and a BPIV3 HN cytoplasmic tail;
- (c) a recombinant RSV G protein comprising a RSV G ectodomain, a HPIV3 HN transmembrane domain, and a HPIV3 HN cytoplasmic tail;
- or
- (d) a recombinant RSV G protein comprising a RSV G ectodomain, a HPIV1 HN transmembrane domain, and a HPIV1 HN cytoplasmic tail;
- wherein the HPIV3 HN gene encodes a HPIV3 HN protein comprising threonine and proline residues at positions 263 and 370, wherein the residue numbering is according to the HPIV3 HN protein sequence set forth as SEQ ID NO:
- 7;
- and
- wherein the recombinant B/HPIV3 is infectious, attenuated, and self-replicating.
Dependencies are inferred from the claim text.