Solid-state nuclear magnetic resonance (ssNMR) method for detecting hydrogen bond structure
AssigneeEast China University of Science and Technology
InventorsShenlin Wang, Sha Zhao
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Abstract
An experimental technology for detecting a hydrogen bond based on an ssNMR technology includes: (1) exciting a 1H nucleus of an RNA sample with a π/2 pulse; (2) applying two π pulses every half rotation period on an X nucleus of the RNA sample; (3) applying a π pulse on the 1H nucleus of the RNA sample; (4) applying two π pulses every half rotation period on the X nucleus of the RNA sample; (5) applying a 90° pulse on 1H and X atoms of the RNA sample; r a chemical shift of X in indirect dimension; (7) applying the 90° pulse on the 1H and X nuclei of the RNA sample; (8) repeating steps 2, 3, and 4; and (9) collecting the 1H signal in direct dimension; where X is selected from the group consisting of 15N and 13C.
Claims20
A solid-state nuclear magnetic resonance (ssNMR) method for detecting a hydrogen bond structure, comprising the following steps:
- (1) exciting a 1H nucleus of an RNA sample with a π/2 pulse;
- (2) applying two π pulses every half rotation period on an X nucleus of the RNA sample;
- (3) applying a n pulse on the 1H nucleus of the RNA sample;
- (4) applying two n pulses every half rotation period on the X nucleus of the RNA sample;
- (5) applying a 90° pulse on 1H and X atoms of the RNA sample;
- (6) recording a chemical shift of X in indirect dimension;
- (7) applying the 90° pulse on the 1H and X nuclei of the RNA sample;
- (8) repeating steps 2, 3, and 4;
- and
- (9) collecting the 1H signal in direct dimension;
- wherein
- X is selected from the group consisting of 15N and 13C.
Dependencies are inferred from the claim text.